中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (11): 796-798.

• 论著 • 上一篇    下一篇

早幼粒细胞白血病基因在寻常性银屑病患者外周血淋巴细胞中表达的研究

李娜1,蓝祖连2,何焱玲3   

  1. 1. 首都医科大学附属北京朝阳医
    2. 北京市朝阳医院皮肤科
    3. 首都医科大学附属北京朝阳医院
  • 收稿日期:2011-12-16 修回日期:2012-07-18 出版日期:2012-11-15 发布日期:2012-10-31
  • 通讯作者: 何焱玲 E-mail:heyanling@medmail.com.cn
  • 基金资助:

    PML基因在维护银屑病基因组稳定性中的作用研究

Expression of promyelocytic leukemia gene in peripheral blood lymphocytes from patients with psoriasis vulgaris

  • Received:2011-12-16 Revised:2012-07-18 Online:2012-11-15 Published:2012-10-31

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摘要：

目的 检测寻常性银屑病患者外周血淋巴细胞中早幼粒细胞白血病（PML）基因的表达，以探索PML基因与银屑病之间的相互关系。方法 收集年龄18 ~ 65岁、未经系统治疗的寻常性银屑病患者50例，健康人对照45例。分离外周血淋巴细胞，使用RNAprep pure 血液总RNA提取试剂盒提取淋巴细胞RNA，用实时荧光定量逆转录聚合酶链反应检测外周血淋巴细胞PML基因mRNA的表达；流式细胞仪检测30例银屑病患者和25例健康人对照外周血淋巴细胞PML蛋白荧光强度值。分别用独立样本t检验对检测结果进行统计分析。结果 50例银屑病患者和45例健康人对照外周血淋巴细胞PML mRNA相对定量（RQ）值分别为14.98 ± 3.64和5.50 ± 1.10，银屑病患者组显著高于健康人对照组（两组比较，t = 16.79，P ＜ 0.05）。30例银屑病患者和25例健康人对照外周血淋巴细胞PML蛋白荧光强度值分别为3.13 ± 0.27和2.43 ± 0.21，银屑病患者组亦显著高于健康人对照组（两组比较，t = 6.93，P ＜ 0.05）。结论 PML基因mRNA及蛋白在银屑病患者外周血淋巴细胞中高表达，提示PML基因可能在银屑病的发病中起一定作用。

关键词: RT-PCR

Abstract:

Objective To detect the expression of promyelocytic leukemia （PML） gene in peripheral blood lymphocytes from patients with psoriasis vulgaris, and to investigate the relationship between PML gene and psoriasis. Methods This study included 50 patients with vulgaris psoriasis who were aged between 18 and 65 years and had never received systemic treatment, as well as 45 healthy controls. Peripheral blood samples were collected from these subjects, and lymphocytes were separated by density-gradient technique. Then, RNA was extracted from the lymphocytes by using RNAprep pure blood kit followed by real time fluorescence-based quantitative reverse transcription-PCR for the detection of PML mRNA expression. Flow cytometry was performed to quantify the expression of PML protein in the lymphocytes. Independent samples t test was carried out to compare the differences in PML expression between the patients and controls. Results The relative quantity （RQ） of PML mRNA was 14.98 ± 3.64 in peripheral blood lymphocytes from the patients, significantly higher than that in the healthy controls （5.50 ± 1.10, t = 16.79, P ＜ 0.05）. Similarly, a significant increment was observed in the fluorescence intensity of PML protein in peripheral blood lymphocytes from the patients compared with the healthy controls （3.13 ± 0.27 vs. 2.43 ± 0.21, t = 6.93, P ＜ 0.05）. Conclusions PML is overexpressed at the protein and mRNA levels in peripheral blood lymphocytes from patients with psoriasis vulgaris, hinting that PML may play a certain role in the development of psoriasis.

Key words: RT-PCR

中图分类号: 

  • R75