中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (11): 825-827.

• 研究报道 • 上一篇    下一篇

UVA联合UVB照射对原代人角质形成细胞增殖的影响

张丽1,2,吴文娟3,起珏3,涂颖4,顾华4,何黎5   

  1. 1. 昆明市第一人民医院
    2. 昆明医科大学第一附属医院皮肤科
    3. 昆明医学院第一附属医院
    4. 昆明医学院第一附属医院皮肤科
    5. 昆明市昆明医学院第一附属医院皮肤科
  • 收稿日期:2011-11-28 修回日期:2012-06-08 出版日期:2012-11-15 发布日期:2012-10-31
  • 通讯作者: 何黎 E-mail:Heli2662@yahoo.com.cn
  • 基金资助:

    云南省应用基础研究重点项目

Objective To observe the effect of ultraviolet irradiation comparising 95% ultraviolet A （UVA） and 5% ultraviolet B （UVB） on the proliferation of human epidermal keratinocytes （HEKs）, in hope to offer a basis for the construction of a photodamaged skin

  • Received:2011-11-28 Revised:2012-06-08 Online:2012-11-15 Published:2012-10-31

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摘要：

目的 探讨95%长波紫外线（UVA）联合5%中波紫外线（UVB）照射对原代人表皮角质形成细胞（HEK）增殖的影响，为构建日光对人工皮肤光损伤模型提供实验依据。方法 用总剂量分别为0、2.5、5、10、20、30、40、60 J/cm2的紫外线（含95%UVA和5%UVB），分别照射体外培养的HEK，继续培养24 h后，用噻唑蓝（MTT）法检测细胞活力，用SPSS软件计算引起HEK半数损伤时的紫外线照射剂量。结果 8种剂量紫外线照射后，HEK相对抑制率分别为0、1.03%、6.60%、17.28%、31.28%、49.59%、59.67%、70.99%，当总照射剂量 ≥ 10 J/cm2时， HEK相对抑制率与对照组相比，差异有统计学意义（F = 62.11，P < 0.05）；HEK相对抑制率为50%时的紫外线照射总剂量为31.31 J/cm2。结论 随着紫外线照射剂量的加大，HEK增殖活力降低，31.31 J/cm2为HEK半数死亡的照射剂量。

Abstract:

Objective To observe the effect of ultraviolet irradiation comparising 95% ultraviolet A （UVA） and 5% ultraviolet B （UVB） on the proliferation of human epidermal keratinocytes （HEKs）, in hope to offer a basis for the construction of a photodamaged skin model induced by sunlight. Methods HEKs were isolated from foreskin tissue and cultured in vitro. After several passages, the HEKs were irradiated with different doses （0, 2.5, 5, 10, 20, 30, 40, 60 J/cm2 ） of UV comprising 95% UVA and 5% UVB. Methyl thiazolyl tetrazolium （MTT） assay was used to evaluate cell viability after 24 hours of additional culture. SPSS １7.0 software was used to calculate the median lethal dose（LD50） of ultraviolet radiation in HEKs. Results The proliferation of HEKs was inhibited by 0, 1.03%, 6.60%, 17.28%, 31.28%, 49.59%, 59.67% and 70.99% respectively after irradiation with UV of 0, 2.5, 5, 10, 20, 30, 40 and 60 J/cm2. A significant inhibition of cell proliferation was observed in HEKs irradiated with UV at a dose of no lower than 10 J/cm2 compared with unirradiated HEKs （F = 62.11, P < 0.05）. The LD50 of UV in HEKs was 31.31 J/cm2. Conclusions Aas the dose of UV irradiation increases, the proliferative activity of HEKs decreases, with the LD50 of UV being 31.31 J/cm2.