中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (10): 750-751.

• 研究报道 • 上一篇    下一篇

人疱疹病毒8型ORF26基因亚型与鳞状细胞癌的相关性研究

李桂寅1,2,冯燕艳3,张德志4,5,吴秀娟2,刘建勇2,5,普雄明2   

  1. 1. 山东省泗水县人民医院
    2. 新疆维吾尔自治区人民医院皮肤性病科
    3. 乌鲁木齐市新疆自治区人民医院皮肤科
    4. 新疆自治区人民医院皮肤性病科
    5.
  • 收稿日期:2010-12-20 修回日期:2012-05-18 出版日期:2012-10-15 发布日期:2012-09-29
  • 通讯作者: 普雄明 E-mail:puxiongming@126.com
  • 基金资助:

    新疆维吾尔自治区自然科学基金

Characterization of human herpesvirus?鄄8 genotypes in squamous cell carcinoma by using the polymorphisms in open reading frame 26 gene

  • Received:2010-12-20 Revised:2012-05-18 Online:2012-10-15 Published:2012-09-29

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摘要：

目的 探讨新疆鳞状细胞癌（简称鳞癌）患者感染人疱疹病毒8型（HHV-8） ORF26基因亚型。方法 巢式PCR扩增41例皮肤鳞癌、46例食管鳞癌石蜡包埋组织标本中HHV-8 ORF26基因，对PCR阳性产物进行双向测序，使用Dnastar软件、Clustal W软件和Phylip软件包对测序结果进行系统发生学分析，从而确定HHV-8 ORF26基因亚型。运用SPSS17.0进行统计学分析。结果 41例皮肤鳞癌组织标本9例HHV-8感染为阳性，阳性率为21.95%；46例食管鳞癌10例为阳性，阳性率为21.74%。新疆皮肤鳞癌患者感染HHV-8 ORF26亚型7例为A亚型，2例为C亚型；食管鳞癌感染HHV-8 ORF26亚型7例为A亚型，3例为C亚型。结论 新疆鳞癌患者感染HHV-8 ORF26亚型属于A亚型和C亚型，A亚型多于C亚型，亚型分布与鳞癌患者的发病部位无关。

关键词: ORF26基因

Abstract:

Objective To analyze the genotypes of human herpesvirus 8 （HHV-8） by using the polymorphisms in open reading frame （ORF） 26 gene in patients with squamous cell carcinoma（SCC）in Xinjiang Uygur Autonomous Region. Methods DNA was extracted from paraffin-embeded tissue specimens from 41 patients with skin SCC and 46 patients with esophagus SCC, and subjected to nested-PCR for the amplification of the ORF26 gene of HHV-8 followed by bidirectional sequencing. Phylogenetic analysis was carried out to determine the genotype of HHV-8 by using the DNASTAR software, Clustal W program, and PHYLIP package. The data were analyzed by SPSS 17.0 software. Results HHV-8 DNA was detected in 9 （21.95%） of 41 skin SCC specimens and 10 （21.74%） of 46 esophagus SCC specimens （?字2 = 0.09, P > 0.05）. As phylogenetic analysis showed, 7 HHV-8 isolates from skin SCC specimens belonged to ORF26 subtype A, 2 subtype C; 7 HHV-8 isolates from esophagus SCC specimens belonged to ORF26 subtype A, and 3 subtype C. Conclusions ORF26 subtype A and C are the predominate genotypes of HHV in patients with SCC in Xinjiang Uygur Autonomous Region, with the prevalence of subtype A higher than that of subtype C. The distribution of HHV subtypes seems unrelated to the location of SCC.

Key words: subtypeORF26