中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (9): 644-646.

• 论著 • 上一篇    下一篇

罗格列酮对HaCaT细胞增殖及β-连环蛋白、细胞周期蛋白D1表达的影响

范妮1,魏志平2,刘彦群2   

  1. 1. 张家港市第一人民医院皮肤科
    2. 徐州医学院附属医院皮肤科
  • 收稿日期:2011-10-10 修回日期:2011-10-21 出版日期:2012-09-15 发布日期:2012-08-31
  • 通讯作者: 魏志平 E-mail:xzwzp1961@yahoo.com.cn

Effects of rosiglitazone on the proliferation of and expressions of β-catenin and cyclin D1 in HaCaT cells

  • Received:2011-10-10 Revised:2011-10-21 Online:2012-09-15 Published:2012-08-31

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摘要：

目的 探讨罗格列酮对体外培养的角质形成细胞株HaCaT细胞体外增殖的影响。方法　用不同浓度的罗格列酮处理HaCaT细胞，四甲基偶氮唑蓝（MTT）法检测HaCaT细胞增殖活性；蛋白免疫印迹法测定HaCaT细胞β-连环蛋白、细胞周期蛋白D1表达水平。 结果 不同浓度的罗格列酮对HaCaT细胞体外增殖均具有抑制作用，药物作用48 h后对HaCaT细胞体外增殖的抑制率分别为18.9%、23.7%、35.1%、44.6%，与溶媒组相比，差异有统计学意义（P < 0.05）。罗格列酮处理HaCaT细胞后，β-连环蛋白、细胞周期蛋白D1表达明显下调。结论 罗格列酮抑制HaCaT细胞的体外增殖的机制可能与β-连环蛋白、细胞周期蛋白D1的表达下调有关。

关键词: Cyclin D1

Abstract:

Objective To estimate the effects of rosiglitazone on cultured HaCaT human keratinocytes and their possible mechanism. Methods HaCaT cells were cultured and treated with different concentrations （10, 20, 40, 80 ?滋mol/L） of rosiglitazone or solvent for 24, 48, 72 and 96 hours, respectively. Cell proliferation was detected with methyl thiazolyl tetrazolium （MTT） assay. Western blot was performed to measure the protein expression of β-catenin and cyclin D1. Results Compared with the solvent-treated cells, the proliferation of HaCaT cells was significantly inhibited by 18.9%, 23.7%, 35.1% and 44.6% （all P < 0.05） after treatment with rosiglitazone of 10, 20, 40 and 80 ?滋mol/L, respectively, for 48 hours. The expressions of β-catenin and cyclin D1 were significantly lower in rosiglitazone-treated HaCaT cells than in solvent-treated cells （all P < 0.05）. Conclusion Rosiglitazone could inhibit the proliferation of HaCaT cells, likely by downregulating the expressions of β-catenin and cyclin D1.

Key words: protein, Cyclin D1

中图分类号: 

  • R758.63