中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (6): 422-425.

• 论著 • 上一篇    下一篇

乙酰肝素酶、基质金属蛋白酶2和金属蛋白酶组织抑制剂2在恶性黑素瘤的表达

陈建立1,张江安2,于建斌3,苏蓓蓓4,孟静5   

  1. 1. 黄河中心医院
    2. 郑州大学第一附属医院
    3. 郑州大学第一附属医院皮肤科
    4. 河南省新乡市中心医院皮肤科
    5. 郑州大学第一附属医院； 商丘市第一人民医院
  • 收稿日期:2011-07-11 修回日期:2011-09-04 出版日期:2012-06-15 发布日期:2012-05-31
  • 通讯作者: 于建斌 E-mail:yjbdoctor@sina.com

Expressions of heparinase, matrix metalloproteinase 2 and tissue inhibitor of metalloproteinase 2 in malignant melanoma

  • Received:2011-07-11 Revised:2011-09-04 Online:2012-06-15 Published:2012-05-31

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摘要：

【摘要】 目的 探讨乙酰肝素酶、基质金属蛋白酶2（MMP2）和金属蛋白酶组织抑制剂2（TIMP2）蛋白在恶性黑素瘤皮损中的表达及意义。方法 选择恶性黑素瘤30例、黑素细胞痣30例、正常皮肤组织15例，分别采用免疫组化SP法检测皮损中乙酰肝素酶、MMP2和TIMP2蛋白的表达。结果 恶性黑素瘤皮损乙酰肝素酶蛋白的阳性表达率与黑素细胞痣、与正常皮肤组织相比，差异均有统计学意义（χ21 = 21.172，P < 0.01；χ22 = 27.805，P < 0.01）。恶性黑素瘤皮损MMP2蛋白的阳性表达率与黑素细胞痣、与正常皮肤组织相比，差异均有统计学意义（χ21 = 19.817，P < 0.01；χ22 = 19.866，P < 0.01）。恶性黑素瘤皮损TIMP2蛋白的阳性表达率与黑素细胞痣、与正常皮肤组织相比，差异均有统计学意义（χ21 = 19.200，P < 0.01；χ22 = 15.000，P < 0.01）。结论 乙酰肝素酶、MMP2和TIMP2蛋白在恶性黑素瘤中的表达水平明显高于其在黑素细胞痣和正常皮肤中的表达水平。

关键词: TIMP2

Abstract:

【Abstract】 Objective To investigate the expressions of heparinase, matrix metalloproteinase 2 （MMP2） and tissue inhibitor of metalloproteinase 2 （TIMP2） in malignant melanoma lesions and their significance. Methods Skin specimens were obtained from the lesions of 30 patients with malignant melanoma, 30 patients with melanocytic nevus and the normal skin of 15 healthy controls. Immunohistochemical SP method was used to detect the protein expression of heparinase, MMP2 and TIMP2. Results The malignant melanoma tissue specimens significantly differed from the melanocytic nevus and control tissue specimens in the expression rate of heparinase （63.33% vs. 6.67% and 0.00, χ2 = 21.172, 27.805, both P < 0.01）, MMP2（70.00% vs. 13.33% and 0.00, χ2 = 19.817, 19.866, both P < 0.01） and TIMP2 （60.00% vs. 6.67% and 0.00, χ2 = 19.200, 15.000, both P < 0.01）. Conclusion The expression of heparinase, MMP2 and TIMP2 is significantly higher in malignant melanoma lesions than in melanocytic nevus lesions and normal skin tissue.

Key words: TIMP2

中图分类号: 

  • R75