中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (5): 366-367.

• 研究报道 • 上一篇    下一篇

系统性红斑狼疮患者外周血单一核细胞Th17相关转录因子RORγt mRNA的表达

白璐1,彭学标2   

  1. 1. 中国人民解放军第89医院皮肤科
    2. 南方医科大学南方医院风湿科
  • 收稿日期:2011-06-22 修回日期:2012-01-05 出版日期:2012-05-15 发布日期:2012-05-03
  • 通讯作者: 彭学标 E-mail:pengxb@fimmu.com

Expression of Th17?鄄related transcription factor RORγt mRNA in peripheral blood mononuclear cells from patients with systemic lupus erythematosus

1,   

  • Received:2011-06-22 Revised:2012-01-05 Online:2012-05-15 Published:2012-05-03

PDF

23

赞

摘要：

目的 探讨Th17细胞在系统性红斑狼疮（SLE）免疫炎症反应中的作用机制。方法 逆转录PCR（RT-PCR）检测12例活动期SLE患者、9例非活动期SLE患者和12例正常人对照PBMC中维A酸相关孤儿核受体γt （RORγt） mRNA表达水平。活动期SLE患者组、非活动期SLE患者组与正常人对照组PBMC中RORγt mRNA表达水平采用近似F检验（Welch方法）和校正的多重比较方法（Dunnett’s T3）进行分析。结果 活动期SLE患者、非活动期SLE患者及正常人对照RORγt mRNA表达水平分别为1.06 ± 0.44，0.65 ± 0.25，0.22 ± 0.08，3组之间差异有统计学意义（F = 23.286，P < 0.01）。其中活动期SLE患者组显著高于非活动期患者组（F = 2.453，P < 0.05）及正常人对照组（F = 6.504，P < 0.05），非活动期SLE患者组显著高于正常人对照组（F = 3.343，P < 0.05）。 SLE患者RORγt mRNA表达水平和SLEDAI之间存在显著正相关（rp = 0.623，P < 0.01）。结论 证实SLE患者存在Th17细胞极化现象，拮抗调控Th17细胞分化的关键转录因子RORγt能减轻SLE的免疫炎症反应而达到治疗SLE的目的。

关键词: RORγt

Abstract:

Objective To investigate the action mechanism of Th17 cells in immunoinflammatory response in systemic lupus erythematosus （SLE）. Methods Reverse-transcription PCR was performed to measure the mRNA expression of the retinoic acid receptor-related orphan nuclear receptor RORγt in peripheral blood mononuclear cells （PBMCs） from 12 patients with active SLE, 9 patients with inactive SLE and 12 normal human controls. Data were statistically analyzed by approximate F test （Welch test） and Dunnett's T3 multiple comparison test （corrected）. Results In the case of RORγt mRNA expression in PBMCs, significant differences existed among the 3 groups （F = 23.286, P < 0.01）; in detail, the patients with active SLE were significantly higher than patients with inactive SLE and normal controls（1.06 ± 0.44 vs. 0.65 ± 0.25, F = 2.453, P < 0.05; 1.06 ± 0.44 vs. 0.22 ± 0.08, F = 6.504, P < 0.05）, and the patients with inactive SLE were significantly increased compared with the normal controls（F = 3.343, P < 0.05）. The expression level of RORγt mRNA was significantly positively correlated with SLE disease activity index （rp = 0.623, P < 0.01）. Conclusions There is a polarization of Th17 cells in patients with SLE. To antagonize the transcription factor RORγt, which plays an essential role in the regulation of Th17 cell differentiation, may facilitate the control of SLE via attenuating the immunoinflammatory response.

Key words: RORγt