中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (4): 228-230.

• 论著 • 上一篇    下一篇

生殖道沙眼衣原体体外经不同浓度阿奇霉素作用后超微结构的变化

蔡宏为1,刘隽华2,陈木开3,李红梅4,蔡壬辛5,6,张勤奋4,6,韩建德7   

  1. 1. 珠海市第二人民医院皮肤科
    2. 中山大学附属第一医院黄埔院区皮肤科
    3. 中山大学附属第一医院
    4. 中山大学有害生物防治国家重点实验室
    5. 中山大学附属第一医院皮肤科
    6.
    7. 广州市中山大学第一医院皮肤科
  • 收稿日期:2011-05-27 修回日期:2011-07-05 出版日期:2012-04-15 发布日期:2012-03-30
  • 通讯作者: 韩建德 E-mail:hanjd_gzb@21cn.net

Ultrastructural changes of genital tract Chlamydia trachomatis after treatment with azithromycin in vitro

  • Received:2011-05-27 Revised:2011-07-05 Online:2012-04-15 Published:2012-03-30
  • Contact: HAN JIANDE E-mail:hanjd_gzb@21cn.net

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摘要：

目的 初步探讨不同浓度阿奇霉素作用下沙眼衣原体的超微结构形态变化。方法 采用McCoy细胞培养方法，在沙眼衣原体实验菌株（D/UW-3/Cx）体外培养过程中添加含阿奇霉素质量浓度为0.0667、0.1340、0.1900、0.2680、0.3330 mg/L的培养液，不加阿奇霉素的培养液作为空白对照组。在透射电镜下观察McCoy细胞和沙眼衣原体超微结构的变化。结果 在接种D/UW-3/Cx时加入阿奇霉素培养48 h后，随阿奇霉素浓度的增加，透射电镜下可见包涵体内外囊泡增多；有异常增大的网状体，部分存在分裂异常，甚至网状体坏死崩解；原体数目减少，体积增大，外膜皱褶增多。阿奇霉素质量浓度为0.3330 mg/L时见不到包涵体。结论 阿奇霉素可引起沙眼衣原体外膜膨大，形成囊泡；网状体异常增大，甚至崩解；原体减少。

关键词: 超微结构

Abstract:

Objective To observe the ultrastructural changes of Chlamydia trachomatis after treatment with azithromycin. Methods The Chlamydia trachomatis laboratory strain （D/UW-3/Cx） was cultured in McCoy cells with or without the presence of azithromycin of 0.0667, 0.1340, 0.1900, 0.2680 and 0.3330 mg/L for 48 hours. The ultrastructural changes of host cells and Chlamydia trachomatis were observed by transmission electron microscopy. Results After 48-hour culture, vesicles increased in number both inside and outside of the inclusion bodies with the rise in azithromycin concentration; there were abnormally large reticulate bodies, some of which experienced abnormal division and even necrosis or breakdown; the number of elementary bodies was decreased, while their size was enlarged, with a more wrinkled outer membrane. No inclusion bodies were seen when the concentration of azithromycin was 0.333 mg/L. Conclusions Azithromycin can induce an increment in the outer membrane of Chlamydia trachomatis, formation of vesicles, abnormal enlargement or breakdown of reticulate bodies, and a decrease in elementary bodies.

Key words: ultrastructure