中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (1): 40-42.

• 研究报道 • 上一篇    下一篇

淋巴毒素β及其受体在变应性接触性皮炎小鼠皮肤中的表达

段瑞1,薛亚东2,栗玉珍3,党林3,王芳1,王丽4   

  1. 1. 哈尔滨医科大学附属二院
    2. 哈尔滨医科大学附属一院
    3. 哈尔滨医科大学附属第二医院皮肤科
    4. 哈尔滨医科大学附属第二医院
  • 收稿日期:2011-04-07 修回日期:2011-06-26 出版日期:2012-01-15 发布日期:2011-12-31
  • 通讯作者: 栗玉珍 E-mail:liyuzhen102@yahoo.com.cn
  • 基金资助:

    哈尔滨市科技创新人才研究专项资金项目

Expression of lymphotoxin-β and its receptor in the skin of a mouse model of allergic contact dermatitis

  • Received:2011-04-07 Revised:2011-06-26 Online:2012-01-15 Published:2011-12-31
  • Contact: li yuzhen E-mail:liyuzhen102@yahoo.com.cn

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摘要：

目的 检测淋巴毒素β及淋巴毒素β受体（LTβR）在变应性接触性皮炎（ACD）小鼠皮肤中的表达。 方法 6 ~ 8周龄雌性BALB/c小鼠随机分为两组，实验组15只和对照组10只，实验组外用2，4-二硝基氟苯建立ACD模型，对照组不行任何处置，采用实时荧光定量PCR和免疫组化的方法分别检测ACD小鼠发生皮炎的耳廓皮肤和对照组小鼠耳廓皮肤中淋巴毒素β mRNA、LTβR mRNA及淋巴毒素β、LTβR蛋白的表达。结果 15只ACD小鼠组织标本淋巴毒素β及LT-βR mRNA相对含量中位数分别为3.630、1.148，其四分位间距分别为1.187、0.617；10只对照组组织标本淋巴毒素β及LTβR mRNA相对含量中位数都为1.000，其四分位间距都为0.000。经秩和检验两组间差异均有统计学意义（P ＜ 0.01）。免疫组化结果显示，ACD小鼠中淋巴毒素β和LTβR表达明显升高，与对照组相比差异亦均有统计学意义（P ＜ 0.01 ）。结论 变应性接触性皮炎小鼠皮损中淋巴毒素β及其受体mRNA相对含量升高，其蛋白表达明显升高。

关键词: 变应性接触性皮炎

Abstract:

Objective To investigate the expression of lymphotoxin-β and its receptor in the skin of a mouse model of allergic contact dermatitis （ACD）. Methods BALB/c female mice at 6 to 8 weeks of age were randomly divided into the experiment group （n = 15） and normal control group （n = 10）. Topical 2, 4-dinitrofluorobenzene （DNFB, 0.5%） was used to establish a model of ACD in the experiment group followed by challenge with DNFB （0.2%） at the right external ear on the 6th day. The normal control mice remained untreated. The right external ear was resected from all of the mice 24 hours after the challenge and divided into 2 parts for the measurement of mRNA and protein expressions of lymphotoxin-β and lymphotoxin-β receptor by fluorescence-based real-time quantitative PCR and immunohistochemistry, respectively. Results The median mRNA expression levels of lymphotoxin-β and lymphotoxin-β receptor were 3.630 and 1.148 respectively in the experimental mice, with the interquartile range being 1.187 and 0.617 respectively, and were both 1.000 in the control mice with the interquartile range being both 0.000. There was a significant difference between the experimental and control group in the mRNA expression levels of lymphotoxin-β and lymphotoxin-β receptor （P < 0.01）. Immunohistochemistry showed a significant increase in the expression of lymphotoxin-β and lymphotoxin-β receptor proteins in the experimental group compared with the control group （P < 0.01）. Conclusion There is an increment in the mRNA and protein expressions of lymphotoxin-β and lymphotoxin-β receptor in the lesions of ACD in mice.

Key words: allergic contact dermatitis