中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (12): 877-879.

• 研究报道 • 上一篇    下一篇

复方甘草酸苷对斑秃患者外周血单一核细胞中γ干扰素、肿瘤坏死因子β表达的调节作用

秦小卫1,陈丽芳2   

  1. 1. 山西省人民医院
    2. 太原市山西省人民医院皮肤科
  • 收稿日期:2011-03-01 修回日期:2011-08-03 出版日期:2011-12-15 发布日期:2011-12-02
  • 通讯作者: 秦小卫 E-mail:qxw0409@163.com

Regulatory effect of glycyrrhizin on the expression of interferon （IFN）-γ and tumor necrosis factor （TNF）-β in peripheral blood mononuclear cells of patients with alopecia areata

  • Received:2011-03-01 Revised:2011-08-03 Online:2011-12-15 Published:2011-12-02

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摘要：

目的 探讨甘草酸苷对斑秃患者外周血单一核细胞（PBMC）γ干扰素（IFN-γ）、肿瘤坏死因子β（TNF-β）表达的调节作用。方法 轻度斑秃患者18例、重症斑秃患者24例及正常人20例为检测对象，用密度梯度离心法常规分离PBMC，用逆转录-聚合酶链反应检测PBMC经植物血凝素和复方甘草酸苷共同刺激后IFN-γ和TNF-β的表达水平。结果 重症斑秃组患者IFN-γ及TNF-β水平均明显高于轻度斑秃组及正常人对照组（P值均 ＜ 0.05），轻度斑秃组高于正常人对照组（P值均 ＜ 0.05）。甘草酸苷与植物血凝素共同刺激斑秃患者PBMC后可下调IFN-γ及TNF-β表达水平（P值均 ＜ 0.05）。结论 甘草酸苷可抑制斑秃患者Th1型细胞因子表达，逆转Th1型反应。

关键词: 肿瘤坏死因子 β

Abstract:

Objective To observe the regulatory effect of glycyrrhizin on the expression of IFN-γ and TNF-β in peripheral blood mononuclear cells （PBMCs） of patients with alopecia areata. Methods PBMCs were obtained from 18 patients with mild alopecia areata, 24 patients with severe alopecia areata and 20 normal human controls, and cultured with phytohemagglutinin （PHA） or the combination of PHA and glycyrrhizin for 24 hours. Then, reverse transcription （RT）-PCR was conducted to detect the mRNA expression of IFN-γ and TNF-β in these cells. Results The mRNA expression levels of IFN-γ and TNF-β in PBMCs were significantly higher in patients with severe alopecia areata than in those with mild alopecia areata and normal human controls （all P < 0.05）, and higher in patients with mild alopecia areata than in normal human controls （both P < 0.05）. A significant decrease was observed in the mRNA expressions of IFN-γ and TNF-β in the PBMCs from patients with alopecia areata after stimulation with the combination of PHA and glycyrrhizin （both P < 0.05）. Conclusion Glycyrrhizin can inhibit the expression of Th1-type cytokines and reverse Th1-type immune response.

Key words: Tumor necrosis factor-β(TNF-β)