中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (9): 661-662.

• 研究报道 • 上一篇    下一篇

盐酸小檗碱抗白念珠菌体外药敏实验研究

竺璐1,胡丽华1,2,徐新美3   

  1. 1. 浙江省皮肤病防治研究所
    2.
    3. 浙江德清浙江省皮肤病防治研究所皮肤科
  • 收稿日期:2010-11-12 修回日期:2011-04-15 出版日期:2011-09-15 发布日期:2011-08-31
  • 通讯作者: 竺璐 E-mail:zulu888@sina.com

In vitro susceptibility testing of Candida albicans to berberine hydrochloride

  • Received:2010-11-12 Revised:2011-04-15 Online:2011-09-15 Published:2011-08-31

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摘要：

目的 探讨盐酸小檗碱（berberine, BBR）对白念珠菌的体外抗真菌活性。方法 采用美国临床实验室标准化委员会（现称美国临床实验室标准化研究所，CLSI）制订的M27-A方案中的酵母菌微量稀释法，测定BBR、氟康唑、BBR联合氟康唑对30株临床分离白念珠菌的抗菌活性。 结果 盐酸小檗碱对白念珠菌MIC值的几何均数（G）为248.18 mg/L。与320、160、80 mg/L BBR联合后，氟康唑MIC值的几何均数从2.64 mg/L降为0.03、0.35、1.35 mg/L。两药对26.7% 的白念菌株有协同抗菌作用，其中包括3株耐氟康唑菌，对56.7%的菌株表现为相加作用，对16.7%的菌株为无关作用，未观察到拮抗作用。结论BBR在体外对白念珠菌具有抗真菌作用并能增加其对氟康唑的敏感性。

关键词: 药敏实验

Abstract:

Objective To test the antifungal activity of berberine（BBR） against Candida albicans in vitro. Methods According to the National Committee for Clinical Laboratory Standards （NCCLS） M27-A protocol, broth microdilution test was performed to assess the susceptibility of 30 clinical isolates of Candida albicans to BBR and fluconazole alone or in combination. Results The geometric mean minimum inhibitory concentration （MIC） was 248.18 mg/L and 2.64 mg/L for BBR and fluconazole alone, respectively. When combined with BBR of 320, 160 and 80 mg/L, the geometric mean MIC decreased to 0.03, 0.35 and 1.35 mg/L respectively for fluconazole. Fluconazole and BBR showed synergistic effects in 26.7% of these isolates including 3 fluconazole-resistant strains, additive effects in 56.7%, indifferent effects in 16.7%, antagonistic effects in none. Conclusion In vitro, BBR has antifungal activity against Candida albicans and can enhance the activity of fluconazole against Candida albicans.

Key words: Susceptibility test