中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (8): 578-580.

• 论著 • 上一篇    下一篇

白介素27基因多态性与广西壮族系统性红斑狼疮的相关性研究

蓝艳1,蒋远文2,2,唐秀生2,2,武洁2,2   

  1. 1. 百色广西右江民族医学院附属医院皮肤科
    2.
  • 收稿日期:2010-10-12 修回日期:2011-03-24 出版日期:2011-08-15 发布日期:2011-07-26
  • 通讯作者: 蓝艳 E-mail:yylanyan@163.com
  • 基金资助:

    广西卫生厅基金资助项目;广西教育厅基金资助项目

Association of interleukin-27 gene polymorphisms with genetic susceptibility to systematic lupus erythematosus in Guangxi Zhuang population

  • Received:2010-10-12 Revised:2011-03-24 Online:2011-08-15 Published:2011-07-26

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摘要：

目的 探讨白介素27（IL-27）基因单核苷酸多态性与广西壮族系统性红斑狼疮（SLE）易感性之间的关系。方法 以135例SLE患者和150例正常人对照者为研究对象，应用聚合酶链反应-限制性片段长度多态性和DNA测序的方法对IL-27基因-964 A/G、2905 T/G单核苷酸多态性进行基因分型。结果 SLE组和正常人对照组中IL-27基因2905 T/G多态性分布差异无统计学意义（χ2 = 1.63，P > 0.05），而IL-27基因-964 A/G多态性的分布差异有统计学意义（χ2 = 9.88，P < 0.01）。等位基因频率的相对风险分析发现，-964 G等位基因携带者患SLE的风险是-964 A等位基因的1.725倍（OR = 1.725，95% CI：1.227 ～ 2.425）。联合基因型分析发现，IL-27的-964 G /2905 G等位基因频率SLE组（10.7%）显著高于对照组（5.3%）（P < 0.01），-964 G/2905 G等位基因携带者显著增加了SLE的发病风险（OR = 2.351，95% CI：1.228 ～ 4.501）。结论 IL-27基因-964 A/G多态性与SLE的发病具有相关性，其中-964 G等位基因可能是SLE的遗传易感基因。

关键词: 基因多态性

Abstract:

Objective To investigate the association between the single nucleotide polymorphisms of interleukin-27 （IL-27） gene and susceptibility to systematic lupus erythematosus （SLE） in Guangxi Zhuang population. Methods In total, 135 patients with SLE and 150 age- and sex-matched human controls of Zhuang nationality were recruited in this study. PCR-restriction fragment length polymorphism （RFLP） analysis and DNA sequencing were performed to analyze the IL-27 gene -964 A/G and -2905 T/G polymorphisms. Results Significant differences were observed in the distribution of IL-27 gene -964 A/G polymorphism （χ2 = 9.88, P < 0.01）. The relative risk for SLE in carriers of G allele at position 964 of IL-27 gene was 1.725 times that in carriers of A allele at this position （OR = 1.725，95% CI: 1.227 - 2.425）. A significant increase was observed in the frequency of 964G/2905G alleles of IL-27 gene in patients with SLE compared with the controls （10.7% vs. 5.3%, P < 0.01）, and the 964G /2905G alleles were associated with a significantly increased risk for SLE （OR = 2.351, 95% CI: 1.228 - 4.501）. Conclusions The IL-27 gene -964 A/G polymorphism is associated with the development of SLE, and the -964 G allele may increase the genetic susceptibility to SLE.

Key words: Gene polymorphism