中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (7): 505-508.

• 论著 • 上一篇    下一篇

遮光剂对小鼠迟发型变态反应的影响

朱慧兰1,赵晓霞2,李润祥3,梁碧华3,黄茂芳3,罗育武3,马少吟3,张锡宝3   

  1. 1. 广州市皮肤防治研究中心
    2. 海口市人民医院
    3. 广州市皮肤病防治所
  • 收稿日期:2010-09-06 修回日期:2011-03-31 出版日期:2011-07-15 发布日期:2011-07-12
  • 通讯作者: 朱慧兰 E-mail:zhlhuilan@hotmail.com
  • 基金资助:

    UVA、UVB对慢性光化性皮炎患者朗格汉斯细胞功能的影响;广州地区人群日晒行为、态度及防护相关因素的调查

Effects of sunscreens on delayed type hypersensitivity in mice

  • Received:2010-09-06 Revised:2011-03-31 Online:2011-07-15 Published:2011-07-12

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摘要：

目的 探讨不同防晒效能遮光剂对小鼠迟发型变态反应的影响和在小鼠免疫保护中的作用。方法 将Balb/c小鼠随机分成5组，每组10只，分别为Ⅰ组：阳性对照组，不予照射；Ⅱ组：单纯模拟太阳辐射（SSR）组；Ⅲ组：遮光剂1组（SPF 15，PPD 12）；Ⅳ组：遮光剂2组（SPF 50，PPD 28）；Ⅴ组：阴性对照组。Ⅱ ~ Ⅴ组均予SSR照射10 d，剂量为1.4 J/cm2 UVA + 100 mJ/cm2 UVB，照射第6天时，Ⅰ、Ⅱ、Ⅲ、Ⅳ组腹腔注射白念珠菌菌丝，并于第10天照射结束后测量所有小鼠双后足足垫，并于足垫内注射白念珠菌菌丝，24 h后测量其厚度，计算出免疫抑制率。Western印迹检测小鼠皮肤中CD1a、CD80、CD86的表达，比较其差异。结果 阳性对照组、遮光剂1组和遮光剂2组小鼠的足垫水肿厚度（分别为0.41 ± 0.38、0.21 ± 0.23、0.30 ± 0.25 mm）明显高于阴性对照组（0.04 ± 0.03 mm）及SSR组（0.14 ± 0.12 mm），P值均 < 0.05，但两种遮光剂组之间差异无统计学意义（P > 0.05）。各组免疫抑制率SSR组为73.0% ± 11.3%，遮光剂1组为54.1% ± 6.4%，遮光剂2组为29.7% ± 7.5%，组间差异有统计学意义（P < 0.01）。Western印迹检测，阳性对照组小鼠皮肤中CD1a蛋白含量明显高于SSR组，阳性对照组和遮光剂1组的CD86均高于SSR组和阴性对照组（P < 0.05），其余各组CD1a、CD80、CD86表达差异无统计学意义（P > 0.05）。结论 亚红斑量紫外线可诱发迟发型变态反应，遮光剂有免疫保护作用，朗格汉斯细胞在紫外线诱导的免疫抑制作用中不是必需的。

关键词: 郎格罕斯细胞

Abstract:

Objective To study the influence of sunscreens with different efficacy on delayed type hypersensitivity （DTH） and their immunoprotective effect in mice. Methods A cohort of mice were randomly divided into 5 groups with 10 mice in each group: group 1 as the positive control without irradiation, group 2 receiving solar-simulated radiation （SSR） only, group 3 receiving SSR and protected by sunscreen 1 with sun protection factor 15 （SPF15） and persistent pigment darkening （PPD） 12, group 4 receiving SSR and protected by sunscreen 2 with SPF 50 and PPD 28, and group 5 as the negative control receiving SSR only. SSR was carried out on the back of mice with the UVA dose being 1.4 J/cm2 and UVB dose being 100 mJ/cm2 for 10 days. After a 5-day irradiation, the groups 1 to 4 were immunized by intraperitoneal injection with 100 μl （107 cells/ml） of Candida albicans suspension. On the 10th day both sides of the posterior foot pad were measured; then the foot pads were injected with additional 50 μl of the Candida albicans suspension. Twenty-four hours after the injection, the thickness of each foot pad was measured, and immunosuppression rate was calculated. Finally, the mice were sacrificed and skin samples were obtained from the back of these mice followed by the examination of CD1a, CD80 and CD86 expression by Western blot. Results The thickness of edema in foot pads was 0.41 ± 0.38 mm, 0.21 ± 0.23 mm and 0.30 ± 0.25 mm in group 1, 3 and 4, respectively, significantly higher than in group 5 and 2（0.04 ± 0.03 mm, 0.14 ± 0.12 mm, respectively, all P < 0.05）, while no significant difference was observed between the group 3 and 4 （P > 0.05）. Significant differences were observed in the immunosuppression rate between group 2, 3 and 4 （73.0% ± 11.3%, 54.1% ± 6.4%, 29.7% ± 7.5%, respectively, all P < 0.01）. Western blot revealed a significant increment in the expression of CD1a protein in group 1 compared with group 2 as well as in the expression of CD86 protein in group 1 and group 3 compared with group 2 and group 5 （all P < 0.05）, but no statistical difference was observed between the other groups in the expression level of CD1a, CD80 or CD86 （P > 0.05）. Conclusions The exposure to sub-erythema dose of UV can induce DTH, and sunscreens have an immunoprotective effect in this process. Epidermal Langerhans cells are not essential for UV-induced immunosuppression.

Key words: Langerhans cells