中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (6): 399-402.

• 论著 • 上一篇    下一篇

己酮可可碱对瘢痕疙瘩成纤维细胞增殖、胶原合成及转化生长因子-β1表达的影响

冯红霞1,辛燕2,商进3,郝玉琴4,金兰兰5   

  1. 1. 内蒙古包钢医院
    2. 包头市内蒙古医学院第三附属医院皮肤科
    3. 包头医学院第一附属医院
    4.
    5. 内蒙古包头市包钢职工医院皮肤科
  • 收稿日期:2010-07-08 修回日期:2010-12-12 出版日期:2011-06-15 发布日期:2011-06-02
  • 通讯作者: 辛燕 E-mail:xinyan69@yahoo.com.cn

Pentoxifylline affects cell proliferation of as well as collagen synthesis and transforming growth factor （TGF）-βl expression by human fibroblasts derived from keloid

  • Received:2010-07-08 Revised:2010-12-12 Online:2011-06-15 Published:2011-06-02

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摘要：

目的 探讨己酮可可碱对瘢痕疙瘩成纤维细胞增殖、胶原合成及转化生长因子（TGF）-β1表达的影响。 方法 取人瘢痕疙瘩及正常皮肤组织培养的第5 ~ 8代成纤维细胞，在含有0.1 ~ 3 g/L己酮可可碱的环境中培养。应用噻唑蓝（MTT）法检测成纤维细胞增殖，双抗体夹心-ELISA法测定TGF-β1表达，RT-PCR检测Ⅰ、Ⅲ型前胶原mRNA表达。结果 0.1 ~ 2 g/L己酮可可碱能明显抑制瘢痕疙瘩和正常皮肤成纤维细胞的增殖，呈明显的量效-时效关系，抑制作用在浓度2 g/L时达到最高。浓度为0. 5 ~ 2 g/L时己酮可可碱能降低瘢痕疙瘩和正常皮肤成纤维细胞TGF-β1表达，1或2 g/L时己酮可可碱能降低瘢痕疙瘩和正常皮肤成纤维细胞Ⅰ、Ⅲ型前胶原mRNA表达。结论 己酮可可碱对瘢痕疙瘩和正常皮肤成纤维细胞的增殖、TGF-β1以及Ⅰ、Ⅲ型前胶原表达均有明显的抑制作用。

关键词: I、III型前胶原mRNA

Abstract:

Objective To investigate the effects of pentoxifylline on the cell proliferation of, collagen synthesis and TGF-βl expression by human fibroblasts derived from keloid. Methods Skin samples were obtained from the lesions of 3 patients with keloid and normal skin of 3 human controls followed by primary culture of fibroblasts. Fibroblasts of 5th to 8th generation were cultured with pentoxifylline of 0.1 to 3 g/L for various durations. Then, MTT assay was performed to detect the cell proliferation of fibroblasts, double antibody sandwich-enzyme linked immunosorbent assay （ELISA） to measure the expression of TGF-βl, and reverse-transcription PCR to examine the mRNA expressions of procollagenⅠand Ⅲ in these fibroblasts. Results The pentoxifylline of 0.1 to 2 g/L markedly inhibited the proliferation of fibroblasts derived from keloid lesions and normal skin, in a dose- and time-dependent manner, with the strongest effect observed in fibroblasts treated with pentoxifylline of 2 g/L. A significant reduction was induced in the TGF-βl mRNA expression in keloid- and normal skin-derived fibroblasts by pentoxifylline of 0.5 to 2 g/L （all P < 0.01）, and in the mRNA expression of procollagenⅠand Ⅲ by pentoxifylline of 1 and 2 g/L （P < 0.05 or 0.01）. Concretely, the relative mRNA expression level of procollagenⅠand Ⅲ was 0.873 ± 0.077, 0.571 ± 0.050 respectively in keloid fibroblasts respectively, and 0.473 ± 0.035, 0.370 ± 0.045 in the control fibroblasts, after treated with pentoxifylline of 1 g/L, 0.750 ± 0.036 and 0.433 ± 0.045 respectively in keloid-derived fibroblasts, 0.390 ± 0.030 and 0.250 ± 0.123 respectively in the control fibroblasts, after treated with pentoxifylline of 2 g/L, significantly lower than that in the keloid-derived （1.216 ± 0.061 and 0.953 ± 0.060） and control （0.836 ± 0.080 and 0.776 ± 0.041） fibroblasts without treatment. Conclusion Pentoxifylline shows an evident suppressive effect on the cell proliferation of, as well as the expression of TGF-βl and procollagenⅠand Ⅲ in fibroblasts derived from keloid lesions and normal skin.

Key words: I, III procollagen mRNA.