绞股蓝皂苷对光损伤BALB/c小鼠皮肤p53、p21蛋白表达的影响

中华皮肤科杂志 ›› 2011, Vol. 44 ›› Issue (4): 253-255.

绞股蓝皂苷对光损伤BALB/c小鼠皮肤p53、p21蛋白表达的影响

袁李梅¹,邓丹琪²,李杨²,刘流³

1. 昆明医学院第二附属医院皮肤病性病/风湿免疫科
2. 昆明医学院第二附属医院
3.

收稿日期:2010-08-04 修回日期:2010-09-22 出版日期:2011-04-15 发布日期:2011-04-01
通讯作者: 邓丹琪 E-mail:danqid@hotmail.com

Effects of gypenosides on the expressions of p53 and p21 protein in photodamaged BALB/c mouse skin

Received:2010-08-04 Revised:2010-09-22 Online:2011-04-15 Published:2011-04-01
Contact: Danqi Deng E-mail:danqid@hotmail.com

摘要/Abstract

摘要：

目的探讨绞股蓝皂苷（GP）抗光损伤的作用机制。方法 80只雌性BALB/c小鼠随机分为8组，每组10只，分别为：空白对照组（未加任何处理）、UVB模型组、GP组Ⅰ（先涂GP后照UVB）、GP组Ⅱ（先照UVB后涂GP）、维生素E组Ⅰ（先涂维生素E乳膏后照UVB）、维生素E组Ⅱ（先照UVB后涂维生素E乳膏）、基质组Ⅰ（先涂基质后照UVB）、基质组Ⅱ（先照UVB后涂基质）。中波紫外线（UVB）照射BALB/c小鼠建立光损伤模型，根据以上分组对光损伤小鼠皮肤进行干预。运用蛋白Western印迹法检测各组小鼠表皮中p53蛋白、p21蛋白的表达。结果 BALB/c小鼠表皮p53蛋白的表达：空白组p53蛋白（0.11 ± 0.08）与UVB模型组（0.22 ± 0.12）相比低表达，GP组Ⅰ（0.44 ± 0.23）高于空白组（P < 0.01），GP组Ⅱ（0.48 ± 0.24）高于空白组（P < 0.01）及UVB模型组（P < 0.05），维生素E组Ⅰ（0.49 ± 0.29）及维生素E组Ⅱ（0.50 ± 0.27）均与GP组作用相似。小鼠表皮p21蛋白的表达各组间差异无统计学意义。结论 1.5% GP乳膏抗光损伤的作用机制之一可能与上调表皮细胞中p53蛋白表达量有关。

关键词: P53肿瘤抑制蛋白

Abstract:

Objective To explore the mechanism underlying the effects of gypenosides （GP） against photodamage. Methods Eighty BALB/c mice were equally divided into 8 groups, i.e., blank control group （untreated）, UVB model group （irradiated with UVB）, GP Ⅰgroup （irradiated with UVB before topical application of GP）, GPⅡ group （irradiated with UVB followed by topical application of GP）, VitEⅠgroup （irradiated with UVB after topical application of Vitamine E cream）, VitEⅡ group （irradiated with UVB followed by topical application of Vitamine E cream）, Vehicle groupⅠ（irradiated with UVB after application of the drug vehicle）, and Vehicle groupⅡ （irradiated with UVB before application of the drug vehicle）. UVB irradiation was performed once every other day for 14 days. Mice were sacrificed after the last irradiation and skin specimens were obtained from the irradiated sites, and the levels of p53 and p21 protein were measured by Western blot in the specimens. Results The expression level of p53 protein was significantly lower in the blank control group than in the UVB model group （0.11 ± 0.08 vs. 0.22 ± 0.12） and GP Ⅰgroup （0.44 ± 0.23, P < 0.01）, in the blank control group and UVB model group than in the GP Ⅱ group （0.48 ± 0.24, P < 0.01, 0.05）. VitEⅠgroup （0.49 ± 0.29） and VitEⅡ group （0.50 ± 0.27） were similar to the GP groups in the expression of p53 protein. No statistical difference was observed in the expression of p21 protein between the eight groups. Conclusion The upregulation of p53 protein expression in epidermal cells may be related to the mechanisms underlying the protective effect of 1.5% GP cream against photodamage.

Key words: tumor suppressor P53 protein

袁李梅邓丹琪李杨刘流. 绞股蓝皂苷对光损伤BALB/c小鼠皮肤p53、p21蛋白表达的影响[J]. 中华皮肤科杂志, 2011, 44(4): 253-255.

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