中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (10): 680-683.

• 论著 • 上一篇    下一篇

地霉菌毛发穿孔试验研究及扫描电镜观察

朱敬先1,王丹2,高顺强1,林元珠3   

  1. 1. 石家庄市河北医科大学第四医院皮肤科
    2. 石家庄河北医科大学第四医院皮肤科
    3. 河北医科大学第四医院皮肤科 050011
  • 收稿日期:2010-02-21 修回日期:2010-04-24 出版日期:2010-10-15 发布日期:2010-10-09
  • 通讯作者: 朱敬先 E-mail:zjxsjz@yahoo.com.cn

In vitro hair perforation test for and scanning electron microscopic observation on Geotrichum

  • Received:2010-02-21 Revised:2010-04-24 Online:2010-10-15 Published:2010-10-09

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摘要：

目的 探讨地霉菌对毛发破坏的程度，比较林生地霉皮损株、林生地霉血液株和白地霉对不同年龄组毛发感染时间及程度的差异。方法 临床采集不同年龄组的健康人毛发，分别进行林生地霉皮损株、林生地霉血液株和白地霉的毛发穿孔试验及扫描电镜观察。结果 林生地霉皮损株、林生地霉血液株和白地霉均可致毛发破坏。在各个年龄组中，林生地霉血液株、林生地霉皮损株和白地霉的毛发穿孔时间差异有统计学意义（P < 0.05）；且感染毛发的时间均随着年龄的增长而延长（P < 0.05）。对不同菌株间的差异进行SNK-q检验，发现在各年龄组中，林生地霉血液株、皮损株和白地霉的毛发穿孔时间两两比较差异均有统计学意义（P < 0.05），林生地霉血液株毛发穿孔时间最短，而白地霉毛发穿孔时间最长，其中林生地霉血液株的毛发穿孔时间范围在53 ~ 64 d，平均为（58.07 ± 3.15） d；林生地霉皮损株的毛发穿孔时间范围在57 ~ 66 d，平均为（61.05 ± 2.55） d；白地霉的毛发穿孔时间范围在61 ~ 74 d，平均为（67.11 ± 3.78） d；对不同年龄组间的差异进行SNK-q检验，发现除 < 2岁组与2 ～ 13 岁组之间比较差异无统计学意义外（P ＞ 0.05），其余各年龄组的两两比较差异均有统计学意义（P < 0.05）。电镜显示，林生地霉和白地霉均能导致毛发破坏，且林生地霉较白地霉对毛发的破坏严重。结论 林生地霉较白地霉对毛发的破坏早且严重。年龄越小，毛发越易受破坏。

关键词: 林生地霉, 皮损株, 血液株, 白地霉, 改良毛发穿孔试验

Abstract:

Objective To determine the degree of damage to hairs by Geotrichum, and to compare the difference in infection duration and intensity by Geotrichum silvicola isolates from skin lesions and blood and Geotrichum candidumn isolates between hairs from different age groups. Methods In vitro hair perforation test was carried out on the hairs from healthy individuals of different age groups. Scanning electron microscopy was performed to observe hairs perforated by Geotrichum silvicola isolates from skin lesions and blood as well as Geotrichum candidumn isolates. Results Both Geotrichum silvicola and Geotrichum candidumn isolates could perforate hairs. The time taken to perorate hairs was significantly different between isolates of Geotrichum silvicola from skin lesions, blood and Geotrichum candidumn in every age groups（all P < 0.05）. The Geotrichum silvicola isolates from blood perforated hairs in the shortest time （range: 53 - 64 days, mean: 58.07 ± 3.15 days）, followed by those from skin lesions （range: 57 - 66 days, mean: 61.05 ± 2.55 days）, and Geotrichum candidumn （range: 61 - 74 days, mean: 67.11 ± 3.78 days）. The time taken to perforate hairs by Geotrichum significantly increased with age, and significant difference was observed between hairs from the four age groups, i.e., < 2 years, 2 - 13 years, 14 - 19 yeas, > 19 years, but not between the age group of < 2 years and that of 2 - 13 years （P > 0.05）. As scanning electron microscopy showed, both Geotrichum silvicola and Geotrichum candidumn could damage hairs, and the degree of damage by Geotrichum silvicola was more severe than that by Geotrichum candidumn. Conclusions The damage to hairs by Geotrichum silvicola is earlier and more severe than that by Geotrichum candidumn. The younger the age, the more liable the hairs to be damaged by Geotrichum.

Key words: Geotrichum Silvicola, Skin Lesion, Blood, Geotrichum candidumn, Hair perforation in vitro