五种常用抗菌药物对泌尿生殖道沙眼衣原体的单独和联合药敏作用

摘要/Abstract

摘要：

目的探讨泌尿生殖道沙眼衣原体D-UW-5/Cx型、E-UW-5/Cx型、G-UW-5/Cx型标准株对阿奇霉素、米诺环素、莫西沙星、多西环素和利福平5种常用抗菌药物体外单独和联合药物敏感性。方法将沙眼衣原体D、E、G型菌株振荡、离心后接种于细胞培养板的McCoy细胞，使McCoy细胞感染率达90％以上后进行药敏试验。采用微量稀释法测定5种抗菌药物体外对沙眼衣原体的单独作用，进而通过棋盘稀释法测定6组抗菌药物联合后的体外相互作用情况。结果在体外，阿奇霉素、莫西沙星、多西环素，米诺环素、利福平的最低抑菌浓度分别为0.25、0.06、0.063 ～ 0.25、0.032 ～ 0.064、0.008 mg/L。阿奇霉素与莫西沙星、多西环素或利福平联合时，其部分抑菌浓度指数均为0.75，对沙眼衣原体有相加作用。而米诺环素与阿奇霉素、莫西沙星或利福平联合时，其部分抑菌浓度指数分别为2.5、2.83、4，相互之间有拮抗作用。结论在体外，阿奇霉素与莫西沙星、多西环素或利福平联用，抗菌活性显著提高。而米诺环素与莫西沙星、多西环素或利福平联合应用时，它们之间的拮抗作用将降低各自的抗菌活性。

关键词: 沙眼衣原体, 抗菌药物, 药敏实验, 药物相互作用

Abstract:

Objective To test the in vitro acitivity of azithromycin, minocyline, moxifloxacin, doxycycline and rifampicin alone or in combination against three standard strains of urogenital Chlamydia trachomatis, i.e. D-UW-5/Cx, E-UW-5/Cx and G-UW-5/Cx. Methods Three standard strains of C. trachomatis were inoculated into McCoy cells, and used to susceptibility testing after more than 90% McCoy cells were infected. Microdilution method was applied to determine the activity of the 5 antimicrobial agents alone, and checkerboard array to determine that in combination. Results The in vitro minimal inhibitory concentrations （MICs） were 0.25 mg/L for azithromycin, 0.06 mg/L for moxifloxacin, 0.063 - 0.25 mg/L for doxycycline, 0.032 - 0.064 mg/L for minocyline, 0.008 mg/L for rifampicin. And, the fractional inhibitory concentration index was constantly 0.75 for the combination of azithromycin with moxifloxacin, doxycycline and rifampicin, 2.5, 2.83 and 4 for the combination of minocyline with azithromycin, moxifloxacin and rifampicin, respectively. Conclusions As far as the activity against C. trachomatis is concerned, there is a synergism for the combination of azithromycin with moxifloxacin, doxycycline and rifampicin, but antagonism for the combination of minocyline with azithromycin, moxifloxacin and rifampicin.

Key words: Chlamydia trachomatis, Antimicrobial, Susceptibility test, Drug interactions

王梅江勇邵丽丽刘原君侯淑萍刘全忠. 五种常用抗菌药物对泌尿生殖道沙眼衣原体的单独和联合药敏作用[J]. 中华皮肤科杂志, 2010, 43(10): 687-690.

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