中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (5): 339-341.

• 论著 • 上一篇    下一篇

TAP-1等位基因多态性与复发性尖锐湿疣间的关系

徐佳1,陆轶凡2,左亚刚3   

  1. 1. 首都医科大学附属北京中医医院
    2. 中国医学科学院 北京协和医学院 北京协和医院
    3. 中国医学科学院北京协和医学院北京协和医院
  • 收稿日期:2009-09-03 修回日期:2010-01-12 出版日期:2010-05-15 发布日期:2012-04-12
  • 通讯作者: 徐佳 E-mail:alice235235@sohu.com
  • 基金资助:

    国家自然科学基金;教育部高等学校博士点基金

Relationship of TAP-1 allele gene polymorphism to recurrent condyloma acuminatum

Yifan Lu2,Ya-gang ZUO   

  • Received:2009-09-03 Revised:2010-01-12 Online:2010-05-15 Published:2012-04-12

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摘要：

目的 探讨TAP-1333、TAP-1637基因多态性与中国人群复发性尖锐湿疣（CA）的关系。方法 采用扩增阻滞突变系统-聚合酶链反应（ARMS-PCR）技术检测88例复发性CA患者和81例正常人对照者TAP-1333基因位点的A/G单核苷酸多态性，以及60例复发性CA患者和60例健康对照者（对照组）TAP-1637基因位点的A/G单核苷酸多态性。结果 CA组TAP-1333等位基因AA、GG、AG基因频率分别为86.36%、0%和13.64%，对照组各等位基因频率分别为79.01%、0%和20.99%，组间差异无统计学意义（χ2 = 1.604，P ＞ 0.05），OR值 ＝ 1.682（0.748 ~ 3.783）。CA组TAP-1637等位基因AA、GG、AG基因频率分别为3.33%、95.00%和1.67%，而对照组各等位基因频率分别为10.00%、60.00%和30.00%,在CA组，天冬氨酸（Asp）杂合型和纯合型出现频率明显低于对照组，而甘氨酸纯合型明显高于对照组，组间差异有统计学意义（χ2 = 23.682，P ＜ 0.01）。AA型：OR值 = 0.310（0.060 ~ 1.604）；GG型：OR值 = 12.667（3.555 ~ 45.135）；AG型：OR值 = 0.04（0.005 ~ 0.308）。结论 TAP-1333位点基因多态性与复发性CA无关；TAP-1637基因位点多态性可能与复发性CA有相关性。

关键词: 等位基因

Abstract:

Objective To investigate the relationship of polymorphisms at codons 333 and 637 of TAP-1 allele gene to recurrent condyloma acuminatum （CA） in a Chinese population. Methods Amplificatory refraction mutation system-PCR （ARMS-PCR） was performed to detect TAP1 polymorphic residues at codon 333 in 88 patients with recurrent CA and 81 age- and sampling date-matched controls and at codon 637 in 60 patients with recurrent CA and 60 age- and sampling date-matched controls. Results The frequencies of AA, GG and AG genotypes at codon 333 of TAP-1 gene were 86.36%, 0, 13.64%, respectively, in patients with recurrent CA, 79.01%, 0 and 20.99%, respectively, in the controls, and no significant difference was observed between the two groups （χ2 = 1.604, P > 0.05）. However, a significant difference was observed in the frequencies of AA, GG and AG genotypes at codon 637 of TAP-1 gene between the patients and controls （3.33% vs 10.0%, 95.00% vs 60.00%, 1.67% vs 30.00%, χ2 = 21.551, P < 0.01）. Conclusions The recurrence of CA may be associated with the polymorphism at codon 637, but not with that at codon 333, of TAP-1 gene.