中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (3): 205-206.

• 技术与方法 • 上一篇    下一篇

实时荧光定量PCR快速鉴定五种常见念珠菌

郭强1,高志祥2,张军东2,王英3,顾军4   

  1. 1. 上海市第二军医大学附属长海医院
    2. 上海 第二军医大学长海医院皮肤科
    3.
    4. 上海第二军医大学附属长海医院皮肤科
  • 收稿日期:2009-04-24 修回日期:2009-06-12 出版日期:2010-03-15 发布日期:2012-03-31
  • 通讯作者: 郭强 E-mail:guoqiang_Y@sohu.com
  • 基金资助:

    白念珠菌转运相关新基因IPF14744的克隆和功能研究

Rapid identification of five common species of Candida with real-time fluorescence-based quantitative PCR

  • Received:2009-04-24 Revised:2009-06-12 Online:2010-03-15 Published:2012-03-31

PDF

442

赞

摘要：

[摘要] 目的 建立实时荧光定量PCR快速检测和鉴定五种常见念珠菌的方法并探讨其可行性。方法 应用真菌通用引物ITS4和ITS86，分别构建五种常见念珠菌重组质粒，进行实时荧光定量PCR检测，绘制融解温度曲线图，并对融解温度进行统计学分析。结果 五种常见念珠菌融解温度为：（87.33±0.13）℃（白念珠菌）、（84.39±0.20）℃（热带念珠菌）、（85.53±0.18）℃（近平滑）、（86.47±0.10）℃（光滑念珠菌）、（91.41±0.18）℃（克柔念珠菌）。结论：本研究显示实时荧光定量PCR方法可快速检测和鉴定五种常见念珠菌。

关键词: 聚合酶链反应

Abstract:

[Abstract] Objective: To establish a Real-time quantitative PCR way in detection and identification of Candida species and explore its feasibility. Method：fungal universal primer ITS4 and ITS86 were designed. Five Candida species were applied to construct recombinant plasmids. Recorded Melting temperature curves after finishing detection by real-time quantitative PCR. Analyzed data. Results: Melting temperatures of five Candida species: (87.33±0.13)℃(Candida albicans), (84.39±0.20)℃(Candida tropicalis), (85.53±0.18)℃(Candida parapsilosis), (86.47±0.10)℃(Candida glabrata), (91.41±0.18)℃(Candida krusei). Conclusion: This study shows the method of Real-time quantitative PCR is a rapid way in detection and identification of Candida species.

Key words: PCR