中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (2): 95-97.

• 论著 • 上一篇    下一篇

生存素、bcl-2在皮肤鳞状细胞癌皮损及细胞系的表达

王珍1,刘梅2,王智敏3,王雅坤4,肖汀5,姜奕4,陈洪铎6,何春涤5   

  1. 1. 沈阳市第七人民医院；中国医科大学附属第一医院
    2. 沈阳市中国医科大学附属第一医院皮肤科
    3.
    4. 沈阳 中国医科大学附属第一医院皮肤科
    5. 中国医科大学附属第一医院皮肤科
    6. 中国医科大学第一附属医院皮肤科
  • 收稿日期:2009-03-12 修回日期:2009-04-09 出版日期:2010-02-15 发布日期:2012-03-30
  • 通讯作者: 王珍 E-mail:drwz1991@163.com
  • 基金资助:
    国家教委归国人员科研启动基金资助项目（编号）;国家自然科学基金资助项目（编号）;省级基金（编号）

Expression of survivin and bcl-2 in human skin squamous cell carcinoma lesions and cell line SCL-1

  • Received:2009-03-12 Revised:2009-04-09 Online:2010-02-15 Published:2012-03-30

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摘要： 目的 探讨凋亡抑制基因生存素、bcl-2在皮肤鳞状细胞癌（简称鳞癌）皮损及皮肤鳞癌细胞系（SCL-1）细胞中的表达意义。方法 细胞免疫组化观察60例鳞癌患者皮损中生存素、bcl－2蛋白的表达并进行统计分析。Western印迹检测HaCaT细胞系和皮肤鳞癌细胞系（SCL-1）细胞中生存素bcl－2蛋白的表达。结果 免疫组化示正常皮肤组织中未见bcl-2与生存素表达；生存素、bcl-2在鳞癌皮损中呈阳性表达，bcl-2、生存素蛋白表达阳性率分别为70%、 60%，两者表达无相关性（P > 0.05）。生存素的表达与年龄、性别、发病部位、病理分级无明显关系，与有无淋巴结转移密切相关。bcl-2蛋白的表达与年龄、性别、发病部位，淋巴结转移无明显关系，但随着鳞癌肿瘤病理分级的升高而降低。Western印迹分析示生存素、bcl-2蛋白在SCL-1细胞中表达明显高于HaCaT细胞。结论 生存素、bcl-2在鳞癌的发生发展中，可能通过不同的抗凋亡路径发挥作用。

关键词: 肿瘤，鳞状细胞, 生存素, bcl-2, 细胞，培养的

Abstract: Objective To investigate the expression of survivin and bcl-2 in human squamous cell carcinoma（SCC） lesions and cell line SCL-1. Methods Tissue samples from 60 patients with SCC and 10 normal human controls were immunohistochemically stained to detect the expressions of survivin and bcl-2. Western blot was used to measure the expressions of bcl-2 and survivin proteins in HaCaT human keratinocytes and SCL-1 human squamous cell carcinoma cells. Results In normal control tissues, there was no expressions of survivin or bcl-2, while in SCC, the expression rates of bcl-2 and survivin were 70% and 60%, respectively, and there was no statistical correlation between the expressions of bcl-2 and survivin （P > 0.05）. Neither the expression of survivin nor that of bcl-2 was correlated to patients′ age, gender or lesional site （all P > 0.05）. A statistical correlation was observed between the pathological stage in patients and expression of bcl-2 as well as between lymph node metastasis and expression of survivin （both P < 0.05）. Western blot analysis revealed a significant increase in the expression of survivin and bcl-2 in SCL-1 cells compared with HaCaT cells. Conclusion In SCC, survivin and bcl-2 seem to play their roles via different anti-apoptotic pathways.