关键词: 贝赫切特综合征;中性白细胞;受体, Fc;过氧化物酶

Abstract: Objective To investigate the mRNA expression of Fc gamma RⅡA （FcγRⅡA） on polymorphonuclear neutrophils （PMN） from patients with Beh?觭et′s disease （BD）. Methods Twenty-five patients with active BD and 20 healthy human controls were included in this study. Blood samples were obtained from all patients with active BD before treatment, from 15 patients with inactive BD after treatment and from healthy controls. PMN were isolated. The FcγRⅡA mRNA expression on PMNs was detected by RT-PCR, and plasma myeloperoxidase （MPO） activity which represented neutrophil activation, was measured spectrophotometrically. Results The relative expression level of FcγRⅡA on PMN and plasma MPO activity were 1.801 ± 0.829 and 32 ± 5 U/L, respectively, in patients with active BD, 0.820 ± 0.625 and 27 ± 4 U/L, respectively, in those with inactive BD, and 0.745 ± 0.931 and 29 ± 5 U/L, respectively, in normal controls; the differences were significant in the two parameters between the patients with active and inactive BD （both P < 0.01）, while no statistical difference was observed between inactive patients and normal human controls （P > 0.05）. There was a positive correlation between the expression level of FcγRⅡA on PMN and plasma MPO activity in patients with BD（r = 0.39, P < 0.01）. Conclusions The mRNA expression of FcγRⅡA on neutrophils is up-regulated in patients with active BD. It is likely that FcγRⅡA is involved in the activation of neutrophils in BD.