中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (11): 723-725.

• 论著 • 上一篇    下一篇

抗原处理相关转运体和MHC-Ⅰ类分子在黑素瘤细胞株的表达

王琳 陶娟 李延 刘业强 杨井 涂亚庭   

  1. 武汉市华中科技大学同济医学院附属协和医院皮肤科 华中科技大学同济医学院附属协和医院皮肤科 武汉华中科技大学同济医学院附属同济医院皮肤科 华中科技大学同济医学院附属协和医院皮肤科
  • 收稿日期:2007-12-13 修回日期:2008-01-26 发布日期:2008-11-15
  • 通讯作者: 涂亚庭 E-mail:yatingtu@yahoo.com.cn

Expression of transporter associated with antigen processing and major histocompatibility complex class-Ⅰ molecule in malignant melanoma cell lines

  

  • Received:2007-12-13 Revised:2008-01-26 Published:2008-11-15

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摘要： 目的 检测抗原处理相关转运体（TAP）及主要组织相容性复合体（MHC）-Ⅰ类分子在恶性黑素瘤细胞系中的表达。方法 采用Western印迹法、RT-PCR和流式细胞仪分别检测TAP蛋白、mRNA和MHC-Ⅰ类分子在3株恶性黑素瘤细胞系（A375、A875、KZ28）中的表达，并与正常黑素细胞做对照。结果 与正常黑素细胞相比，TAP mRNA表达在A375、A875、KZ28细胞系中均显著降低，t值分别为5.89，4.45和4.57，P值均 < 0.01；TAP 蛋白的表达均明显降低，t值分别为5.46，4.32和4.67，P值均 < 0.01。在A375细胞中TAP mRNA和蛋白下降最显著。MHC-Ⅰ类分子也具有相同的趋势，t值分别为6.16，5.22和5.61，P值均 < 0.01。结论 TAP蛋白及其mRNA在A375、A875、KZ28细胞系中表达显著降低，可能为恶性黑素瘤细胞逃逸机体免疫监视的一条途径。

关键词: 黑色素瘤;肿瘤细胞, 培养的;基因, MHCⅠ类

Abstract: Objective To explore the expression of transporter associated with antigen processing （TAP） and major histocompatibility complex class （MHC）-Ⅰ molecule in malignant melanoma cell lines. Methods Three malignant melanoma cell lines, including A375, A875, and KZ28 cells as well as normal melanocytes were cultured. Western blot, reverse transcription PCR and flow cytometry were used to detect the protein and mRNA expression of TAP as well as the membrane expression of MHC-Ⅰin these cells. Results A significant decrease was observed in the expression of TAP mRNA （t = 5.89, 4.45 and 4.57 respectively, all P < 0.01） and protein （t = 5.46, 4.32 and 4.67 respectively, all P < 0.01） in A375, A875 and KZ28 cells compared with the melanocytes, with the strongest decrease occurring in A375 cells. Similarly, the expression of MHC-Ⅰ molecule was significantly lower in A375, A875 and KZ28 cells than that in the melanocytes （t = 6.16, 5.22 and 5.61 respectively, all P < 0.01）. Conclusions The protein and mRNA expression of TAP is down-regulated in three melanoma cell lines A375, A875 and AZ28, which may contribute to the escape of melanoma cells from human immune surveillance.