中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (10): 677-679.

• 论著 • 上一篇    下一篇

尖锐湿疣组织LTBP-1和TGF-βRⅡ的表达

胡勇 郭庆 曾凡钦 唐增奇 林燕辉 汤红峰   

  1. 南方医科大学附属顺德第一人民医院 广州市中山大学第二医院皮肤科 广州市中山大学第二医院皮肤科 广州市中山大学附属第二医院皮肤科
  • 收稿日期:2007-11-09 修回日期:2008-06-06 发布日期:2008-10-15
  • 通讯作者: 郭庆 E-mail:guoqingzsy@163.com

Expression of latent transforming growth factor beta binding protein-1 and transforming growth factor beta typeⅡreceptor in condyloma acuminatum tissues

HU Yong   

  • Received:2007-11-09 Revised:2008-06-06 Published:2008-10-15

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摘要： 目的 探讨潜在结合蛋白（LTBP）-1及其Ⅱ型受体（TGF-βRⅡ）与尖锐湿疣发病的相关性。方法 采用实时荧光定量PCR技术和SP免疫组化技术分别检测30例CA皮损及17例正常包皮组织中LTBP和TGF-βRⅡ的mRNA和蛋白的表达。结果 CA皮损中LTBP-1及TGF-βRⅡ的mRNA的表达水平均低于对照组包皮组织（2 -△△Ct ＞ 2）。免疫组化结果显示：LTBP-1和TGF-βRⅡ的染色强度较正常人包皮组织降低，差异有统计学意义（P ＜ 0.01）。 结论 LTBP-1和TGF-βRⅡ的表达下调，可导致TGF-β活化及信号转导通路的异常。

关键词: 尖锐湿疣;转化生长因子β;潜在转化生长因子β结合蛋白;Ⅱ型受体, 转化生长因子β

Abstract: Objective To investigate the role of the expression of latent transforming growth factor beta binding protein-1 （LTBP-1） and transforming growth factor beta receptor typeⅡ（TGF-beta RⅡ） in the pathogenesis of condyloma acuminatum （CA）. Methods Samples were resected from the lesions of 30 patients with CA and prepuces of 17 normal human controls. The mRNA and protein expressions of LTBP-1 and TGF-betaRⅡ were assessed by quantitative real-time PCR and a streptavidin-biotin-peroxidase staining technique, respectively. Results As shown by Real time PCR, the mRNA expression levels of LTBP-1 and TGF-betaRⅡ were significantly higher in CA tissues than those in the controls, with the average value of 2 （-Delta Delta Ct ） being 2.46 and 3.43, respectively. A lower intensity of stainning was observed for LTBP-1 and TGF-betaRⅡ in CA tissues compared with the normal controls（182.51 ± 9.89 vs 167.78 ± 12.56, 187.35 ± 11.23 vs 170.15 ± 13.21, t = 5.62, 3.70 respectively, both P < 0.01). Conclusion The decrease in the expression of both LTBP-1 and TGF-betaRⅡ may lead to the abnormality in the activation of TGF-beta and signal transduction pathways.

Key words: Condyloma acuminatum, Transforming growth factor beta, Latent TGFβbinding protein, TypeⅡreceptor,transforming growth factor beta