中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (9): 582-584.

• 论著 • 上一篇    下一篇

甲基结合蛋白MeCP2与mbd2在SLE患者中的表达

伍洲炜 吴瑞勤 郑捷 徐金华 施伟民   

  1. 上海市第一人民医院 上海交通大学附属第一人民医院皮肤科 上海交通大学医学院附属瑞金医院皮肤科 上海市复旦大学附属华山医院皮肤科 上海同济大学附属同济医院皮肤科
  • 收稿日期:2007-11-22 修回日期:2007-12-21 发布日期:2008-09-15
  • 通讯作者: 伍洲炜 E-mail:johnwuzhouwei@yahoo.com.cn

Gene expression of methyl-CpG-binding protein 2 and methyl-CpG-binding domain 2 in patients with systemic lupus erythematosus

  

  • Received:2007-11-22 Revised:2007-12-21 Published:2008-09-15

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摘要： 目的　探讨甲基结合蛋白MeCP2及去甲基化酶mbd2基因的表达异常在SLE发病中的作用。方法　以RT-PCR方法检测SLE缓解期、活动期患者与正常人外周血单一核细胞（PBMC）中MeCP2及mbd2基因的表达水平，并进行相关性分析。结果　SLE活动期、缓解期及正常人对照组PBMC中MeCP2的表达差异无统计学意义（P ＞ 0.05）。SLE缓解期患者PBMC中mbd2的表达水平显著高于正常人对照组（t = 12.8，P ＜ 0.01）；SLE活动期患者PBMC中mbd2表达显著高于SLE缓解期（t = 20.0，P ＜ 0.01）。SLE患者PBMC中mbd2的表达与SLEDAI评分呈正相关（r = 0.737，P = 0.0001）。正常人对照组PBMC中mbd2的表达与MeCP2的表达呈正相关（r = 0.550，P = 0.0222）。结论　在正常情况下，MeCP2和mbd2的表达相互制约，在SLE患者中这种关系被破坏。

关键词: 系统性红斑浪疮, MeCP2, mbd2;

Abstract: Objective To investigate the gene expression of methyl-CpG-binding protein 2 （MeCP2） and methyl-CpG-binding domain 2 （mbd2） in patients with systemic lupus erythematosus（SLE） and their significance in the pathogenesis of SLE. Methods Semiquantitative reverse transcriptase-PCR was applied to detect the mRNA expression of MeCP2 and mbd2 in PBMCs obtained from relieved （n = 17）, active （n = 17） SLE patients and healthy controls （n = 17）. The correlations were further analyzed among these parameters. Results No significant difference was observed in the expression level of MeCP2 mRNA among active SLE patients, relieved SLE patients and healthy controls （P > 0.05）. The expression of mbd2 in relieved SLE patients was significantly higher than that in health controls （t = 12.8, P < 0.01), but lower than that in active SLE patients（t = 20.0, P < 0.01）. The expression of mbd2 positively correlated with SLEDAI （r = 0.737, P = 0.0001） of patients with SLE, and a positive correlation was also observed between the expression of mbd2 and MeCP2 in healthy controls（r = 0.550, P = 0.0222）. Conclusions The expression of MeCP2 and mbd2 may be mutually constrained in normal human, but this relationship seems to be disturbed in patients with SLE.