中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (5): 285-287.

• 论著 • 上一篇    下一篇

尖锐湿疣中转化生长因子β受体、受体活化Smad和共有Smad的表达

尚进 何威 杨进清 李颖 何云志 吴军 任莉 付秋红   

  1. 第三军医大学第二附属医院（新桥医院）皮肤科 第三军医大学第二附属医院（新桥医院）皮肤科 重庆第三军医大学新桥医院皮肤科 第三军医大学附属新桥医院皮肤科 400037
  • 收稿日期:2007-10-30 修回日期:2007-11-13 发布日期:2008-05-15
  • 通讯作者: 尚进 E-mail:shangjin1998@163.com

Expression of TGFβ receptors, receptor-activated Smads and common-partner Smad in condyloma acuminatum

  

  • Received:2007-10-30 Revised:2007-11-13 Published:2008-05-15

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摘要： 目的 探讨尖锐湿疣中转化生长因子β（TGF-β）信号转导途径TGFβ受体（TGFβR）、受体活化Smad和共有Smad蛋白的表达情况。方法 采用EliVisionTMplus免疫组织化学技术分别检测20例尖锐湿疣和15例正常人对照皮肤中TGFβRⅠ、TGFβRⅡ、Smad1/2/3、磷酸化Smad2/3（p-Smad2/3）和Smad4的表达。结果 TGFβRⅠ、TGFβRⅡ、Smad1/2/3、p-Smad2/3和Smad4在对照正常人皮肤的表皮中均有表达。尖锐湿疣表皮中TGFβRⅠ、TGFβRⅡ、Smad1/2/3、p-Smad2/3和Smad4的免疫组化染色强度均明显低于对照正常人皮肤的表皮（P < 0.05或P < 0.01）。结论 尖锐湿疣表皮中存在着TGFβR、受体活化Smad和共有Smad蛋白的表达下调或缺失，可干扰TGFβ信号向下游的转导。失去TGFβ抑制作用的表皮角质形成细胞可出现异常增殖，导致尖锐湿疣表皮过度增殖病理改变的形成。

关键词: 尖锐湿疣, 转化生长因子β, 转化生长因子β受体, Smad, 信号转导

Abstract: Objective To investigate the expressions of transforming growth factor beta receptors（TGF beta R）, receptor-activated Smads and common-partner Smad in condylomata acuminata. Methods Tissue samples were collected from 20 patients with condylomata acuminate and 15 normal human controls. EliVisionTM plus immunohistochemical technique was used to detect the distribution and expression of TGF beta RⅠ, TGF beta RⅡ, Smad1/2/3, phosphorylated Smad2/3 and Smad4 in condylomata acuminata and normal control skin. Results Positive immunohistochemical staining for TGFβRⅠ, TGFβRⅡ, Smad1/2/3, p-Smad2/3 and Smad4 was detected in the epidermis of normal control skin. The intensity of immunohistochemical staining was significantly lower for TGFβRⅠ, TGFβRⅡ, Smad1/2/3, p-Smad2/3 and Smad4 in the epidermis of condyloma acuminatum than in that of normal control skin （P < 0.05 or < 0.01）. Conclusion The expressions of TGF beta R, receptor-activated Smads and common-partner Smad are decreased or absent in the epidermis of condylomata acuminata, which might interfere with TGF beta/Smad signaling and contribute to the development of epidermal hyperplasia in condylomata acuminata.

Key words: condyloma acuminatum, TGFβ, TGFβR, Smad, signal transduction