中华皮肤科杂志 ›› 2020, Vol. 53 ›› Issue (7): 554-556.doi: 10.35541/cjd.20190705

• 研究报道 • 上一篇    下一篇

Fe3O4纳米酶对白念珠菌的体外抑制实验研究

王昊珏1,房灵2,崔成军3,施启丰4,陆胜5,陆显义1,高利增6   

  1. 1. 无锡市锡山人民医院（东南大学附属中大医院无锡分院）
    2. 无锡市锡山人民医院
    3. 江苏省无锡锡山人民医院 东南大学附属中大医院无锡分院
    4.
    5. 江苏省锡山市人民医院皮肤科
    6. 扬州大学医学院
  • 收稿日期:2019-06-28 修回日期:2019-12-09 发布日期:2020-07-06
  • 通讯作者: 高利增 E-mail:lzgao@yzu.edu.cn
  • 基金资助:
    国家自然科学基金;无锡市卫生计生科研项目;无锡市科技发展指导性计划项目

In vitro inhibitory effect of Fe3O4 nanozymes against Candida albicans

  • Received:2019-06-28 Revised:2019-12-09 Published:2020-07-06
  • Supported by:
    National Natural Science Foundation of China;Scientific Research Project of Wuxi Health and Family Planning Commission;Wuxi Science and Technology Development Guiding Project

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摘要： 【摘要】 目的 研究Fe3O4纳米酶对白念珠菌的抗菌作用。方法 改进水热合成法，制备Fe3O4纳米酶。以0.5 g/L Fe3O4纳米酶和0.1% H2O2与菌液共培养，分为纳米酶组、H2O2组、联合组（纳米酶 + H2O2共处理），以未做处理菌液为对照组。4组菌液以沙氏液体培养基培养，每隔2 h检测600 nm处吸光度（A值），观察白念珠菌生长情况。取4组菌液共处理2 h，扫描电镜观察各组白念珠菌形态；涂板后，于36 ℃培养48 h，观察菌落形成情况并计数，计算抑菌率。多组间均数比较采用单因素方差分析，组间两两比较采用LSD-t检验。结果 对照组白念珠菌具有相对稳定的生存曲线，而纳米酶组、H2O2组、联合组白念珠菌的生长均受到抑制。4组菌落计数分别为124 830 ± 45 170、86 330 ± 13 960、91 670 ± 31 370、30 330 ± 3010，差异有统计学意义（F = 9.41，P < 0.05），联合组低于对照组（t = 4.63，P < 0.05）。H2O2组、纳米酶组、联合组抑菌率分别为30.84% ± 5.00%、26.57% ± 11.24%、75.70% ± 2.42%，差异有统计学意义（F = 9.413，P < 0.01），联合组高于H2O2组、纳米酶组（t = 8.08、4.27，P < 0.01），差异均有统计学意义。扫描电镜观察，经过处理后菌体形态发生皱缩、破裂甚至崩解等改变。结论 Fe3O4纳米酶联合H2O2对白念珠菌抑菌效果明显。

关键词: 念珠菌病, 白色念珠菌, 过氧化氢, 过氧化氢酶, 磁性纳米粒子, Fe3O4

Abstract: 【Abstract】 Objective To assess antimicrobial activity of Fe3O4 nanozymes against Candida albicans. Methods Fe3O4 nanozymes were prepared by using a modified hydrothermal synthesis method. Candida albicans suspensions were divided into 4 groups: nanozyme group treated with 0.5 g/L Fe3O4 nanozymes, H2O2 group treated with 0.1% H2O2 , combination group treated with 0.5 g/L Fe3O4 nanozymes and 0.1% H2O2 , and control group receiving no treatment. Candida albicans in the above 4 groups was all cultured with Sabouraud liquid medium, the absorbance （A value） at 600 nm was detected every 2 hours, and the growth of Candida albicans was observed. After 2-hour treatment, the morphology of Candida albicans in the 4 groups was observed by scanning electron microscopy; after plate coating, Candida albicans was cultured at 36 ℃ for 48 hours, colonies were observed and counted, and the inhibition rate of colony formation was calculated. One-way analysis of variance was used for comparing means among several groups, and least significant difference （LSD）-t test for multiple comparisons. Results A relatively stable survival curve of Candida albicans was observed in the control group, while the growth of Candida albicans was inhibited in the nanozyme group, H2O2 group and combination group. The colony count significantly differed among the control group, H2O2 group, nanozyme group and combination group （124 830 ± 45 170, 86 330 ± 13 960, 91 670 ± 31 370 and 30 330 ± 3010 respectively; F = 9.41, P < 0.05）, and was significantly lower in the combination group than in the control group （t = 4.63, P < 0.05）. There was a significant difference in the antimicrobial rate among the H2O2 group, nanozyme group and combination group （30.84% ± 5.00%, 26.57% ± 11.24%, 75.70% ± 2.42% respectively; F = 9.413, P < 0.01）, and the combination group showed a significantly higher inhibition rate of colony formation compared with the H2O2 group and nanozyme group （t = 8.08, 4.27 respectively, both P < 0.01）. Scanning electron microscopy showed changes in the morphology of Candida albicans treated with H2O2 and nanozymes alone or in combination, including shrinkage, rupture and even collapse. Conclusion Fe3O4 nanozymes combined with H2O2 have obvious antimicrobial effect against Candida albicans.

Key words: Candidiasis, Candida albicans, Hydrogen peroxide, Catalase, Magnetite nanoparticles, Ferumoxytol

引用本文

王昊珏 房灵 崔成军 施启丰 陆胜 陆显义 高利增. Fe3O4纳米酶对白念珠菌的体外抑制实验研究[J]. 中华皮肤科杂志, 2020,53(7):554-556. doi:10.35541/cjd.20190705