中华皮肤科杂志 ›› 2019, Vol. 52 ›› Issue (3): 172-175.doi: 10.3760/cma.j.issn.0412-4030.2019.03.005

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输入性疖肿型皮肤蝇蛆病一例及致病瘤蝇COⅠ基因序列分析

张瑾1    王玉兰2    滕新栋1    徐翮飞1    岳巧云3    朱可1    陈晓光1   

  1. 1山东国际旅行卫生保健中心，青岛 266071； 2日照国际旅行卫生保健中心，山东日照 276800； 3中山出入境检验检疫局技术中心，广东中山 528403
  • 收稿日期:2018-07-05 修回日期:2018-12-14 发布日期:2019-03-04
  • 通讯作者: 张瑾 E-mail:zhjoohyn@hotmail.com
  • 基金资助:
    国家质检总局科技项目（2017IK115）

A case of imported furuncular cutaneous myiasis and cytochrome oxidaseⅠ gene sequence analysis of the pathogenic Cordylobia anthropophaga

Zhang Jin1, Wang Yulan2, Teng Xindong1, Xu Hefei1, Yue Qiaoyun3, Zhu Ke1, Chen Xiaoguang1   

  1. 1Shandong International Travel Health Care Center, Qingdao 266071, China; 2Rizhao International Travel Health Care Center, Rizhao 276800, Shandong, China; 3Technology Center, Zhongshan Entry⁃Exit Inspection and Quarantine Bureau, Zhongshan 528403, China
  • Received:2018-07-05 Revised:2018-12-14 Published:2019-03-04
  • Contact: Zhang Jin E-mail:zhjoohyn@hotmail.com
  • Supported by:
    Scientific Research Project of National Quality Supervision, Inspection and Quarantine Administration （2017IK115）

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摘要： 【摘要】 报道1例输入性疖肿型皮肤蝇蛆病，并对致病瘤蝇的线粒体细胞色素C氧化酶亚基Ⅰ（COⅠ）基因序列进行分析。患者女，33岁。患者于1个月前去非洲加纳和喀麦隆两地，期间未采取任何防蚊措施，且有室外晾晒衣物经历。皮肤科检查：左上臂内侧和左侧胸部外侧各见一疖性隆起，直径1 ~ 2 cm，鲜红色，边缘不规则，触之较硬，皮损表面中央有小孔。形态学观察显示皮损处挤出的虫体疑似为蝇蛆。PCR扩增虫体COⅠ基因片段获得650 bp左右的产物，测序后经BLAST比对分析显示，该产物与喀麦隆2010年分离的嗜人瘤蝇COⅠ基因FR719158.1亲缘关系最近，序列相似性为99.84%，在系统进化树上聚集在一起。综合患者的临床特征、旅行史和致病瘤蝇的序列分析结果，确认该病例为嗜人瘤蝇导致的输入性疖肿型皮肤蝇蛆病。

关键词: 皮下蝇蛆病, 蝇科, 电子传递复合物Ⅳ, 疖肿型皮肤蝇蛆病, 嗜人瘤蝇

Abstract: 【Abstract】 To report a case of imported furuncular cutaneous myiasis, and to analyze the sequence of the mitochondrial cytochrome C oxidase subunitⅠ （COⅠ） gene of the pathogenic Cordylobia anthropophaga. A 33-year-old female patient had a travel history to Ghana and Cameroon in Africa 1 month prior to the presentation. No anti-mosquito measures were taken during her stay, and she hung up the laundries outside to dry for several times. Skin examination showed furuncular protuberances with diameters of 1 - 2 cm on the inner side of the left upper arm as well as on the outer side of the left chest, which were bright red and hard on palpation with irregular borders and a small hole on their central surface. Morphological identification revealed that the larva squeezed from the lesion was suspected as myiasis. After PCR amplification of the COⅠ gene of the larva, an about 650-bp PCR product was acquired. Sequencing and BLAST analysis showed that this product was most closely related to the COⅠ gene （GenBank accession number: FR719158.1） of Cordylobia anthropophaga isolated in Cameroon in 2010 with the sequence similarity being 99.84%, and they were grouped together on the phylogenetic tree. According to the clinical features and travel history of the patient and the sequencing results of the pathogenic Cordylobia anthropophaga, this case was confirmed as imported furuncular cutaneous myiasis caused by Cordylobia anthropophaga.

Key words: Hypodermyiasis, Muscidae, Electron transport complex Ⅳ, Furuncular cutaneous myiasis, Cordylobia anthropophaga

引用本文

张瑾 王玉兰 滕新栋 徐翮飞 岳巧云 朱可 陈晓光. 输入性疖肿型皮肤蝇蛆病一例及致病瘤蝇COⅠ基因序列分析[J]. 中华皮肤科杂志, 2019,52(3):172-175. doi:10.3760/cma.j.issn.0412-4030.2019.03.005

Zhang Jin, Wang Yulan, Teng Xindong, Xu Hefei, Yue Qiaoyun, Zhu Ke, Chen Xiaoguang. A case of imported furuncular cutaneous myiasis and cytochrome oxidaseⅠ gene sequence analysis of the pathogenic Cordylobia anthropophaga[J]. Chinese Journal of Dermatology, 2019, 52(3): 172-175.doi:10.3760/cma.j.issn.0412-4030.2019.03.005