中华皮肤科杂志 ›› 2018, Vol. 51 ›› Issue (1): 39-42.doi: DOI：10.3760/cma.j.issn.0412-4030.2018.01.010

• 论著 • 上一篇    下一篇

进展期非节段型白癜风患者外周血CD4+ T细胞亚群分析

甄昱1,李珊山2   

  1. 1. 吉林大学第一医院皮肤科
    2. 长春市吉林大学第一医院皮肤科
  • 收稿日期:2017-03-28 修回日期:2017-06-05 出版日期:2018-01-15 发布日期:2018-01-11
  • 通讯作者: 李珊山 E-mail:shansalee@163.com
  • 基金资助:

    国家自然科学基金

Analysis of CD4+ T cell subsets in the peripheral blood of patients with progressive non-segmental vitiligo

Yu ZHEN1,   

  • Received:2017-03-28 Revised:2017-06-05 Online:2018-01-15 Published:2018-01-11
  • Supported by:

    National Natural Science Foundation of China

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摘要：

目的 探讨进展期非节段型白癜风（NSV）患者外周血中CD4+ T细胞各亚群的免疫状态。方法 用Ficoll密度梯度离心方法，收集30例进展期NSV患者以及30例健康对照外周血中单个核细胞。流式细胞仪检测CD4+ T细胞各亚群细胞因子。ELISA方法检测血清CD4+ T细胞亚群细胞因子的分泌水平。结果 在进展期NSV组外周血CD4+ T细胞中，Th1与Th17比例均显著高于健康对照［（8.68 ± 4.54）%比（5.64 ± 3.83）%；（1.96 ± 1.07）%比（1.17 ± 0.6）%， P＜0.05 ］；而Th2与Treg比例与健康对照相比，差异无统计学意义（均P > 0.05）。Th1/Treg和Th17/Treg比例都显著高于健康对照（1.32 ± 0.23比2.38 ± 0.28，P＜0.01；0.29 ± 0.12比0.41 ± 0.24，P＜0.05），而Th2/Treg比例则无显著变化。患者血清中干扰素（IFN）?γ、IL?4与转化生长因子（TGF）?β1与健康对照相比，差异无统计学意义（均P > 0.05），而IL?17A的含量显著高于健康对照［（23.08 ± 5.80） pg/ml 比（8.66 ± 1.83） pg/ml，P＜0.05］。结论 进展期NSV外周血CD4+ T细胞亚群中，效应性T细胞Th1和Th17占据主导地位，与抑制性T 细胞Treg之间比例失衡，可能是白癜风自身免疫反应的因素之一。

Abstract:

Zhen Yu, Li Shanshan Department of Dermatology, First Hospital of Jilin University, Changchun 130021, China Corresponding author: Li Shanshan, Email:shansalee@163.com 【Abstract】 Objective To explore the immune status of CD4+ T cell subsets in the peripheral blood of patients with progressive non-segmental vitiligo （NSV）. Methods Mononuclear cells were isolated from the peripheral blood of 30 patients with progressive NSV and 30 healthy controls by using Ficoll density gradient centrifugation. Flow cytometry was performed to determine the proportions of CD4+ T cell subsets. Enzyme-linked immunosorbent assay （ELISA） was conducted to detect serum levels of cytokines secreted by CD4+ T cell subsets. Results The proportions of Th1 and Th17 cells in the peripheral blood were significantly higher in the patients with progressive NSV than in the healthy controls（Th1 cells: 8.68% ± 4.54% vs. 5.64% ± 3.83%, P < 0.05; Th17 cells: 1.96% ± 1.07% vs. 1.17% ± 0.6%, P < 0.05）, while there were no significant differences in the proportions of Th2 or Treg cells between the patients and healthy controls （both P > 0.05）. The patients with progressive NSV also showed significantly higher ratios of Th1/Treg and Th17/Treg compared with the healthy controls （Th1/Treg ratio: 1.32 ± 0.23 vs. 2.38 ± 0.28, P < 0.01; Th17/Treg ratio: 0.29 ± 0.12 vs. 0.41 ± 0.24, P < 0.05）, while the ratio of Th2/Treg did not differ between the patients and healthy controls. There were no significant differences in serum levels of interferon （IFN）-γ, interleukin （IL）-4 and transforming growth factor （TGF）-β1 between the patients and healthy controls （all P > 0.05）, while the serum level of IL-17A was significantly higher in the patients than in the healthy controls（23.08 ± 5.80 pg/mL vs. 8.66 ± 1.83 pg/mL, P < 0.05）. Conclusions Effector Th1 and Th17 cells are the dominant CD4+ T cell subsets in the peripheral blood of patients with progressive NSV, and the imbalance between the effector and suppressor （Treg） CD4+ T cell subsets may be one of the factors for autoimmune response in patients with vitiligo.