中华皮肤科杂志 ›› 2017, Vol. 50 ›› Issue (7): 508-511.

• 论著 • 上一篇    下一篇

特应性皮炎患者血清转谷氨酰胺酶2特异性IgE水平与病情相关性研究

苏惠春1,2,孙婧3,罗阳4,韩悦5,刘笑纯5,张警兮5,温禾3,姚煦6,王宝玺7   

  1. 1. 福建医科大学附属第一医院
    2. 中国医学科学院北京协和医学院皮肤病研究所博士
    3. 中国医学科学院皮肤病研究所
    4. 中国医学科学院南京皮肤病研究所
    5. 中国医学科学院北京协和医学院皮肤病研究所
    6. 南京 中国医学科学院北京协和医学院皮肤病研究所
    7. 中国医学科学院北京协和医学院整形外科医院
  • 收稿日期:2016-10-09 修回日期:2017-04-05 发布日期:2017-07-03
  • 通讯作者: 姚煦 E-mail:dryao_xu@126.com
  • 基金资助:
    国家自然科学基金；2014年亚美医学基金会（MMAAP）皮肤病项目基金资助；江苏省自然科学基金;国家自然科学基金；2014年亚美医学基金会（MMAAP）皮肤病项目基金资助；江苏省自然科学基金;国家自然科学基金；2014年亚美医学基金会（MMAAP）皮肤病项目基金资助；江苏省自然科学基金

Relationship between the serum level of transglutaminase 2?specific IgE and disease condition in patients with atopic dermatitis

  • Received:2016-10-09 Revised:2017-04-05 Published:2017-07-03
  • Supported by:
    1 Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China 2 Institute of Dermatology, The First Affiliated Hospital of Fujian Medical University ,Fuzhou,350004 3 Institute of Dermatology, The Plastic Surgery Hospital ，Chinese Academy of Medical Sciences; 1 Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China 2 Institute of Dermatology, The First Affiliated Hospital of Fujian Medical University ,Fuzhou,350004 3 Institute of Dermatology, The Plastic Surgery Hospital ，Chinese Academy of Medical Sciences; 1 Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China 2 Institute of Dermatology, The First

PDF

38

摘要： 目的 检测特应性皮炎（AD）患者血清中转谷氨酰胺酶2（TG2）特异性IgE（sIgE）的表达水平，并分析与患者病情的相关性。方法 共入组77例AD患者，其中，44例 ≥ 12岁，33例 < 12岁，内源性AD（特异性sIgE阴性，且总IgE < 150 kU/L）20例，外源性AD（一种以上的外源性过敏源sIgE++以上，或总IgE ≥ 150 kU/L）49例。采用免疫捕获及生物素标记的酶联免疫分析法检测77例AD患者、40例成人寻常性银屑病患者和30例成人健康对照血清中TG2 sIgE水平。记录AD患者年龄、病程、SCORAD评分、嗜酸性粒细胞计数和总IgE及TG2的sIgE水平。结果 ≥ 12岁AD组、 < 12岁AD组、银屑病组和对照组外周血TG2 sIgE水平（A450）分别为1.02 ± 0.2、1.04 ± 0.044、0.93 ± 0.25、0.71 ± 0.13。≥ 12岁AD、寻常性银屑病和健康对照组外周血中TG2 sIgE表达水平差异有统计学意义（χ2 = 37.407，P < 0.001）；两两组间比较发现， ≥ 12岁AD组、银屑病组TG2 sIgE水平均显著高于对照组（t值分别为7.38、4.83，均P < 0.001）。内源性AD组TG2 sIgE水平（1.16 ± 0.03）高于外源性AD组（1.02 ± 0.20）（t = 2.27，P = 0.02）。AD患者组TG2 sIgE水平与年龄、病程、SCORAD评分、嗜酸性粒细胞计数、血清总IgE水平均无显著相关性（r值分别为0.03、0.14、-0.04、-0.08和0.06，均P > 0.05）。结论 AD患者血清中TG2 sIgE水平明显升高，TG2可能是AD患者的一种自身抗原，但TG2 sIgE与患者的病情严重程度无明显相关性。

Abstract: Su Huichun, Sun Jing, Luo Yang, Han Yue, Liu Xiaochun, Zhang Jingxi, Wen He, Yao Xu, Wang Baoxi Department of Allergy and Rheumatology, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China （Su HC［current affiliation: Department of Dermatology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, China］, Sun J［current affiliation: Department of Dermatology, Peking University People′s Hospital］, Luo Y, Han Y, Liu XC, Zhang JX, Wen H, Yao X）; Department of Dermatology, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100144, China （Wang BX） Corresponding authors: Yao Xu, Email: dryao xu@126.com; Wang Baoxi, Email: wangbx@ncstdlc.org 【Abstract】 Objective To detect the serum level of transglutaminase 2 （TG2）?specific IgE （sIgE） in patients with atopic dermatitis （AD）, and to analyze its correlation with the disease condition. Methods A total of 77 patients with AD were enrolled into this study, including 44 patients aged ≥ 12 years and 33 patients aged < 12 years. Of the 77 patients, 20 were diagnosed with intrinsic AD, which was characterized by the absence of sIgE and total serum IgE values < 150 kU/L, and 49 with extrinsic AD characterized by positive （++） or even strongly positive sIgE for more than 1 kind of exogenous allergens, or total serum IgE values ≥ 150 kU/L. Immunocapture?biotinylated detector enzyme immunoassay was performed to detect the serum level of TG2?sIgE in 77 patients with AD, 40 adult patients with psoriasis vulgaris （PV） and 30 healthy adult controls. Clinical data on the AD patients were recorded, including age, disease duration, SCORAD score, blood eosinophil count, levels of total IgE and TG2?sIgE. Results The serum levels of TG2?sIgE in AD patients aged ≥ 12 years, AD patients aged < 12 years, PV patients and healthy controls were 1.02 ± 0.2, 1.04 ± 0.044, 0.93 ± 0.25 and 0.71 ± 0.13, respectively. Additionally, the serum level of TG2?sIgE significantly differed among AD patients aged ≥ 12 years, PV patients and healthy controls （χ2 = 37.407, P < 0.001）, and was significantly higher in both AD patients aged ≥ 12 years and PV patients than in the healthy controls （t = 7.38, 4.83, respectively, both P < 0.001）. Moreover, the intrinsic AD group showed significantly higher TG2?sIgE levels compared with the extrinsic AD group （1.16 ± 0.03 vs. 1.02 ± 0.20, t = 2.27, P = 0.02）. The TG2?sIgE level was uncorrelated with the patients′ age, disease duration, SCORAD score, blood eosinophil count or serum total IgE levels in AD patients （r = 0.03, 0.14, -0.04, -0.08, 0.06, respectively, all P > 0.05）. Conclusion The serum level of TG2?sIgE obviously increases in AD patients, so TG2 may be one kind of autoantigen in AD patients, but there is no significant correlation between the TG2?sIgE level and AD severity.

引用本文

苏惠春 孙婧 罗阳 韩悦 刘笑纯 张警兮 温禾 姚煦 王宝玺. 特应性皮炎患者血清转谷氨酰胺酶2特异性IgE水平与病情相关性研究[J]. 中华皮肤科杂志, 2017,50(7):508-511. doi: