伊曲康唑、特比萘芬和他克莫司单用及联合对皮炎外瓶霉的体外抗真菌作用

中华皮肤科杂志 ›› 2017, Vol. 50 ›› Issue (4): 283-285.

伊曲康唑、特比萘芬和他克莫司单用及联合对皮炎外瓶霉的体外抗真菌作用

何承彦¹,孙毅²,高露娟³,李明⁴,曾同祥²

1. 长江大学
2. 荆州市中心医院
3. 复旦大学附属中山医院
4. 上海市中山医院皮肤科

收稿日期:2016-08-12 修回日期:2016-08-29 出版日期:2017-04-15 发布日期:2017-03-31
通讯作者: 高露娟 E-mail:gao_lujuan@163.com
基金资助:
国家自然科学基金;复旦大学附属中山医院优秀青年医师计划

In vitro antifungal activity of tacrolimus alone or in combination with itraconazole or terbinafine against Exophiala dermatitidis

¹, ²,Lu-juan GAO ⁴, ⁴

Received:2016-08-12 Revised:2016-08-29 Online:2017-04-15 Published:2017-03-31
Contact: Lu-juan GAO E-mail:gao_lujuan@163.com
Supported by:
National Natural Science Foundation

摘要/Abstract

摘要： 目的探讨他克莫司与伊曲康唑、特比萘芬联合对皮炎外瓶霉的体外抗真菌效果。方法参考美国临床实验室标准化研究所M38?A2方案，测定特比萘芬和伊曲康唑对12株皮炎外瓶霉的最低抑菌浓度；利用棋盘法，测定他克莫司和伊曲康唑或特比萘芬的联合抗皮炎外瓶霉效果。结果特比萘芬和伊曲康唑对皮炎外瓶霉最低抑菌浓度范围分别为（0.06 ~ 0.125） mg/L和（0.5 ~ 1） mg/L。他克莫司和特比萘芬联合对5株皮炎外瓶霉、他克莫司和伊曲康唑联合对10株皮炎外瓶霉有协同作用。两组均无拮抗作用。结论他克莫司在体外与伊曲康唑或特比萘芬联合应用时，能够增加皮炎外瓶霉对伊曲康唑和特比萘芬的敏感性。

Abstract: He Chengyan, Sun Yi, Gao Lujuan, Li Ming, Zeng Tongxiang Department of Dermatology, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou 434100, Hubei, China （He CY, Sun Y, Zeng TX）; Department of Dermatology, Zhongshan Hospital, Fudan University, Shanghai 200032, China （Gao LJ, Li M） Corresponding authors: Gao Lujuan, Email: gao_lujuan@163.com; Sun Yi, Email: jzzxyysy@163.com 【Abstract】 Objective To evaluate in vitro antifungal activity of tacrolimus combined with itraconazole or terbinafine against Exophiala dermatitidis （E. dermatitidis）. Methods The minimum inhibitory concentrations （MICs） of itraconazole and terbinafine against 12 strains of E. dermatitidis were determined using the Clinical and Laboratory Standards Institute （CLSI） broth microdilution susceptibility method （M38?A2 Document）. A broth microdilution checkerboard method was used to evaluate the in vitro antifungal activity of tacrolimus combined with itraconazole or terbinafine against E. dermatitidis. Results The MIC ranges of terbinafine and itraconazole against E. dermatitidis were 0.060 - 0.125 mg/L and 0.5 - 1 mg/L, respectively. The combination of tacrolimus with terbinafine showed synergistic inhibitory effects against 5 strains of E. dermatitidis, while the combination of tacrolimus with itraconazole revealed synergistic effects against 10 strains of E. dermatitidis. No antagonism was observed in either of the two combinations. Conclusion In vitro combination of tacrolimus with itraconazole or terbinafine can enhance the antifungal activity of itraconazole or terbinafine against E. dermatitidis.

何承彦孙毅高露娟李明曾同祥. 伊曲康唑、特比萘芬和他克莫司单用及联合对皮炎外瓶霉的体外抗真菌作用[J]. 中华皮肤科杂志, 2017, 50(4): 283-285.doi:

Lu-juan GAO. In vitro antifungal activity of tacrolimus alone or in combination with itraconazole or terbinafine against Exophiala dermatitidis[J]. Chinese Journal of Dermatology, 2017, 50(4): 283-285.doi:

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