中华皮肤科杂志 ›› 2017, Vol. 50 ›› Issue (3): 195-198.

• 论著 • 上一篇    下一篇

4-羟苯基维胺在不同载体中对人瘢痕疙瘩成纤维细胞增殖及凋亡影响

李周娜1,金哲虎2,陈香儒1   

  1. 1. 延边大学附属医院皮肤科
    2. 吉林省延吉 延边大学附属医院皮肤科
  • 收稿日期:2016-12-02 修回日期:2016-12-05 发布日期:2017-03-01
  • 通讯作者: 金哲虎 E-mail:jinzh_621@163.com
  • 基金资助:
    超声联合4HPR微泡载体治疗瘢痕疙瘩的实验研究;超声介导的4-HPR药物对瘢痕疙瘩治疗作用;4-羟苯基维胺对增生性瘢痕成纤维细胞的影

Effects of 4?hydroxyphenyl retinamide in different vehicles on the proliferation and apoptosis of human keloid fibroblasts

Ju Na-Lee LEEJu Na1, 1   

  • Received:2016-12-02 Revised:2016-12-05 Published:2017-03-01

PDF

22

摘要： 目的 探讨4?羟苯基维胺（4?HPR）在不同载体中对人瘢痕疙瘩成纤维细胞（HKF）增殖及凋亡的影响。方法 采用薄膜-超声分散法制备4?HPR脂质体溶液及4?HPR微泡溶液。用不同浓度（0 ~ 80 mg/L）4?HPR脂质体溶液作用原代HKF 6 ~ 48 h后，噻唑蓝（MTT）法检测细胞增殖情况。将部分HKF分成3组，分别用15 mg/L 4?HPR溶液、4?HPR脂质体溶液、4?HPR微泡处理，每组再分成两个亚组，一个亚组在给药后接受超声处理，另一亚组不做超声处理。作用24 h后，噻唑蓝（MTT）法检测HKF的增殖情况。流式细胞仪检测15 mg/L 4?HPR脂质体或4?HPR微泡处理24 h后HKF的凋亡情况。结果 成功制备4?HPR脂质体溶液及4?HPR微泡溶液。MTT检测显示，4?HPR脂质体溶液在1 ~ 80 mg/L浓度范围内对HKF增殖有抑制作用，且增殖抑制率与药物浓度呈正相关（r = 0.633，P < 0.01）。超声处理后4?HPR微泡组与4?HPR溶液及4?HPR脂质体组对HKF的增殖抑制作用差异均有统计学意义（P < 0.01）。4?HPR脂质体组和4?HPR微泡组HKF凋亡率分别为（21.81 ± 3.73）%和（39.79±1.61）%，较对照组 （6.18 ± 0.61）%均显著增加（均P < 0.01）。结论 成功制备4?HPR微泡，不同载体中4?HPR可促进HKF凋亡，进而抑制增殖，其中4?HPR微泡结合超声抑制作用较4?HPR脂质体强。

Abstract: Li Zhouna, Chen Xiangru, Jin Zhehu Department of Dermatology, Yanbian University Hospital, Yanji 133000, Jilin, China Corresponding author: Jin Zhehu, Email: jinzh_621@163.com 【Abstract】 Objective To evaluate effects of 4?hydroxyphenyl retinamide （4?HPR） in different vehicles on the proliferation and apoptosis of human keloid fibroblasts （HKFs）. Methods A film?ultrasonic dispersion method was used to prepare 4?HPR liposome solution and 4?HPR microbubbles. Primary HKFs were in vitro treated with the 4?HPR liposome solution at different concentrations of 0 - 80 mg/L for 6 - 48 hours, and the proliferative activity of HKFs was evaluated by methyl thiazolyl tetrazo?lium （MTT） assay. Some other HKFs were divided into 3 experimental groups to be treated with 15 mg/L 4?HPR solution （4?HPR solution group）, 15 mg/L 4?HPR liposome solution （4?HPR liposome solution group） and 15 mg/L 4?HPR microbubbles （4?HPR microbubble group）, respectively, and each group was divided into ultrasonic?treated and ?untreated subgroups. HKFs without treatment served as control group. After 24?hour treatment, MTT assay was conducted to evaluate the proliferative activity of HKFs in the above groups, flow cytometry to detect apoptosis of HKFs in all groups except the 4?HPR solution group. Results The 4?HPR liposome solution and 4?HPR microbubbles were successfully prepared. MTT assay showed inhibitory effects of 4?HPR liposome solution at concentrations of 1 - 80 mg/L on the proliferation of HKFs, and the proliferation inhibition rate was positively associated with the drug concentrations （r = 0.633, P < 0.01）. After the ultrasonic treatment, inhibitory effects on the proliferation of HKFs significantly differed among the 4?HPR microbubble group, 4?HPR solution group and 4?HPR liposome solution group （P < 0.01）. The 4?HPR liposome solution group and the 4?HPR microbubble group both showed significantly increased apoptosis rates （21.81% ± 3.73%, 39.79% ± 1.61%, respectively） compared with the control group （6.18% ± 0.61%, both P < 0.01）. Conclusion The 4?HPR microbubbles are successfully prepared, and 4?HPR in different vehicles all can promote HKF apoptosis and suppress HKF proliferation, among which, 4?HPR microbubbles in combination with ultrasonic treatment have stronger inhibitory effects than the 4?HPR liposome solution.

引用本文

李周娜 金哲虎 陈香儒. 4-羟苯基维胺在不同载体中对人瘢痕疙瘩成纤维细胞增殖及凋亡影响[J]. 中华皮肤科杂志, 2017,50(3):195-198. doi:

Ju Na-Lee LEEJu Na. Effects of 4?hydroxyphenyl retinamide in different vehicles on the proliferation and apoptosis of human keloid fibroblasts[J]. Chinese Journal of Dermatology, 2017, 50(3): 195-198.doi: