中华皮肤科杂志 ›› 2017, Vol. 50 ›› Issue (2): 109-112.

• 论著 • 上一篇    下一篇

带状疱疹患者疱液及外周血病毒载量与血浆S100β和神经元特异性烯醇化酶水平的研究

张馨月1,钟婷婷2,钟建桥1,许飏3   

  1. 1. 泸州医学院附属医院皮肤科
    2. 四川省泸州市泸州医学院附属医院皮肤科
    3. 四川省泸州医学院附属医院皮肤科
  • 收稿日期:2016-03-18 修回日期:2016-08-23 出版日期:2017-02-15 发布日期:2017-01-24
  • 通讯作者: 许飏 E-mail:xxff2007@163.com
  • 基金资助:
    带状疱疹患者脑损伤的研究及与PHN的关系

Correlation of plasma levels of S100β protein and neuron?specific enolase with viral load in vesicle fluid and peripheral blood of patients with herpes zoster

  • Received:2016-03-18 Revised:2016-08-23 Online:2017-02-15 Published:2017-01-24
  • Contact: Yang XU E-mail:xxff2007@163.com

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摘要： 目的 检测带状疱疹患者疱液、血液水痘-带状疱疹病毒（VZV）DNA载量及血浆S100β蛋白、神经元特异性烯醇化酶（NSE）浓度变化，并探讨其相关性。方法 在西南医科大学附属医院皮肤科收集50例急性期带状疱疹住院患者疱液、治疗前后外周血标本和20例健康对照外周血标本。采用实时荧光定量PCR检测疱液及外周血病毒载量， ELISA检测血浆S100β蛋白、NSE浓度。结果 50例带状疱疹患者疱液中均检测到VZV；治疗前18例、治疗后5例血液中测出VZV。对照组血液中均未测出VZV。急性期带状疱疹患者疱液病毒载量与血浆S100β、NSE含量均呈正相关（r = 0.535、0.430，均P < 0.05）。治疗前血VZV阳性患者较血VZV阴性患者血浆S100β、NSE水平升高（均P < 0.05），血VZV DNA载量与血浆S100β、NSE均呈正相关（r = 0.711、0.645，均P < 0.05）。结论 带状疱疹患者可检测出VZV DNA，且急性期带状疱疹患者疱液、治疗前血VZV DNA载量越高，血浆S100β、NSE水平越高。

Abstract: Zhang Xinyue, Zhong Tingting, Zhong Jianqiao, Xu Yang Department of Dermatology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan, China （the current affiliation of the first author was Department of Dermatology, University-Town Hospital of Chongqing Medical University, Chongqing 401331, China） Corresponding author: Xu Yang, Email: xxff2007@163.com 【Abstract】 Objective To detect the varicella-zoster virus （VZV） DNA load in vesicle fluid and peripheral blood, as well as plasma levels of S100β protein and neuron-specific enolase （NSE） in patients with herpes zoster before and after treatment, and to explore their correlations. Methods Vesicle fluid samples were collected before treatment, and peripheral blood samples before and after treatment from 50 inpatients with acute herpes zoster in the Department of Dermatology of the Affiliated Hospital of Southwest Medical University, and peripheral blood samples were also obtained from 20 healthy controls. Real-time fluorescence-based quantitative PCR （qRT-PCR） was performed to determine the viral load in the vesicle fluid and peripheral blood samples, and enzyme-linked immunosorbent assay （ELISA） to detect the plasma levels of S100β protein and NSE. Results VZV DNA was present in all the vesicle fluid samples, as well as in peripheral blood samples from 18 patients before treatment and 5 patients after treatment, but not found in any of the healthy controls. Positive correlation was found between the viral load in vesicle fluid and plasma levels of S100β protein and NSE （r = 0.535, 0.430, respectively, both P < 0.05） in the patients with acute herpes zoster. Before treatment, patients with VZV DNA in peripheral blood showed significantly increased plasma levels of S100β protein and NSE compared with those without （both P < 0.05）, and the viral load in peripheral blood was positively correlated with plasma levels of S100β protein and NSE （r = 0.711, 0.645, respectively, both P < 0.05）. Conclusion VZV DNA is present in some patients with herpes zoster, and increased VZV DNA loads in the vesicle fluid and peripheral blood are related to elevated plasma levels of S100β protein and NSE before treatment.