Abstract:

Wang Xiaopo, Wen Sijian, Su Qianya, Song Hao, Ni Nana, Jiang Yiqun, Chen Hao, Sun Jianfang Department of Pathology，Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China（Wang XP, Wen SJ, Song H, Jiang YQ, Chen H, Sun JF）；Department of Cosmetic Laser Surgery （Su QY）; Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing 210042, China（Ni NN） Corresponding author: Sun Jianfang, Email: fangmin5758@aliyun.com 【Abstract】 Objective To measure the of casitas B?lineage lymphoma b （Cbl?b） in cutaneous malignant melanoma （CMM） tissues and cell lines A375, M14 and MV3, and to assess its clinical significance. Methods Immunohistochemistry was carried out to measure Cbl?b in paraffin?embedded tissue sections from 69 cases of CMM and 30 cases of pigmented nevus. Real?time fluorescence?based quantitative PCR and Western?blot analysis were performed to determine the mRNA and protein s of Cbl?b in melanocytes and the three cell lines respectively. Results The positive rate of Cbl?b was significantly higher in CMM tissues than in pigmented nevus tissues （75.36% ［52/69］ vs. 13.33% ［4/30］, χ2 = 32.745, P < 0.01）. The level of Cbl?b in CMM tissues was positively correlated with the stage of tumor progression, Clark′s level and Breslow thickness of CMM （rs = 0.569, 0.654, 0.727, respectively, all P < 0.01）. As fluorescence?based quantitative PCR showed, there were significant differences in the mRNA of Cbl?b among A375, M14 and MV3 cells （F = 176.537, P < 0.01）. Western?blot analysis revealed the protein of Cbl?b in the 3 melanoma cell lines and melanocytes, which was highest in A375 cells, and lowest in melanocytes. Conclusion Cbl?b was overexpressed in CMM tissues and cell lines.