氟芬那酸丁酯抑制紫外线诱导皮肤急性光毒性作用的研究

中华皮肤科杂志 ›› 2015, Vol. 48 ›› Issue (2): 104-107.

氟芬那酸丁酯抑制紫外线诱导皮肤急性光毒性作用的研究

郭建美¹,仲少敏²,陶荣¹,苗晓琳¹,吴艳²

1. 北京大学第一医院
2. 北京大学第一医院皮肤科

收稿日期:2014-03-30 修回日期:2014-07-16 发布日期:2015-01-28
通讯作者: 吴艳 E-mail:adelewu@medmail.com.cn

Inhibitory effect of butyl flufenamate on ultraviolet-induced acute skin phototoxicity

Received:2014-03-30 Revised:2014-07-16 Published:2015-01-28
Contact: 艳吴 E-mail:adelewu@medmail.com.cn

摘要/Abstract

摘要：

目的探讨非甾体类抗炎药氟芬那酸丁酯（BT）对紫外线诱导皮肤急性光毒性反应的抑制作用及可能的作用机制。方法 SKH-1无毛小鼠背部随机编号分为6组，仅照光组、BT + 照光组、照光 + BT组、基质 + 照光组、照光 + 基质组、空白组。在处理后24 h收集各组皮肤标本，HE染色，用实时PCR检测caspase 3、p53、COX-2、PGER1、IL-1β、IL-6的表达量以及免疫荧光检测caspase 3的表达量。结果照光后24 h，与仅照光组相比，涂抹BT软膏组皮肤水肿程度减轻，凋亡细胞的数目减少。实时PCR显示：与空白组相比，仅照光组caspase 3、p53、COX-2、PGER1、IL-1β、IL-6均有升高（P < 0.05）；BT软膏 + 照光组与仅照光组比较，caspase 3、p53、COX-2、PGER1、L-1β、IL-6表达明显下调（P < 0.05），照光 + BT软膏组仅caspase 3、p53的表达明显下调（P < 0.05）。免疫荧光检测：仅照光组与空白组相比caspase 3表达明显上调；与仅照光组比较，BT软膏 + 照光组和照光 + BT软膏组caspase 3表达显著下降。结论 BT可以部分抑制紫外线对于SKH-1无毛小鼠皮肤造成的急性光毒作用。

Abstract:

Guo Jianmei, Zhong Shaomin, Tao Rong, Miao Xiaolin, Wu Yan. Department of Dermatology, Peking University First Hospital, Beijing 100034, China Corresponding author: Wu Yan, Email: adelewu@medmail.com.cn 【Abstract】 Objective To evaluate the inhibitory effect of butyl flufenamate （BT） on ultraviolet （UV）-induced acute skin phototoxic reaction, and to investigate its possible mechanisms. Methods Eight SKH-1 hairless mice were included in this study. The back of each SKH-1 hairless mouse was divided into six regions, which were then randomly classified into six groups: blank group receiving no treatment, UV group receiving UV radiation only, BT + UV group and vehicle + UV group topically treated with BT ointment and vehicle respectively followed by UV radiation, UV + BT group and UV + vehicle group topically treated with BT ointment and vehicle respectively after UV radiation. Skin samples were obtained from these mice at 24 hours after treatment. Subsequently, hematoxylin-eosin （HE） staining was performed, real-time PCR was carried out to detect mRNA expressions of caspase-3, p53, COX-2, PGER1, interleukin （IL）-1β, IL-6, and an immunofluorescence assay was conducted to observe the expression of caspase-3. Statistical analysis was carried out by repeated-measures analysis of variance （ANOVA）. Results Compared with the UV group, both BT + UV group and UV + BT group showed a decrease in the degree of skin edema and number of apoptotic cells at 24 hours after UV radiation. Real-time PCR showed that the mRNA expressions of caspase-3, p53, COX-2, PGER1, IL-1β and IL-6 were significantly higher in the UV group than in the blank group （all P < 0.05）, but significantly lower in the BT + UV group than in the UV group （all P < 0.05）, and only the expressions of caspase-3 and p53 mRNAs were significantly decreased in the UV + BT group compared with the UV group （both P < 0.05）. The immunofluorescence assay revealed that the expression of caspase-3 increased in the UV group compared with the blank group, but decreased in both BT + UV group and UV + BT group compared with the UV group. Conclusion BT could partially inhibit UV-induced acute skin phototoxicity in SKH-1 hairless mice.

郭建美仲少敏陶荣苗晓琳吴艳. 氟芬那酸丁酯抑制紫外线诱导皮肤急性光毒性作用的研究[J]. 中华皮肤科杂志, 2015,48(2):104-107. doi: