中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (9): 611-614.

• 论著 •    下一篇

淋球菌rpsE基因突变与大观霉素耐药性的研究

朱文1,蒋法兴2,苏晓红3,乐文静4,王娜5   

  1. 1. 安徽医科大学附属省立医院皮肤科
    2. 安徽省立医院皮肤科
    3. 南京 中国医学科学院北京协和医学院皮肤病研究所
    4. 中国医学科学院皮肤病研究所
    5. 安徽省立医院
  • 收稿日期:2013-10-21 修回日期:2014-05-28 出版日期:2014-09-15 发布日期:2014-09-01
  • 通讯作者: 蒋法兴 E-mail:jiangfxing@126.com
  • 基金资助:
    安徽省自然科学基金

Mutations in the rpsE gene and spectinomycin resistance in Neisseria gonorrhoeae

  • Received:2013-10-21 Revised:2014-05-28 Online:2014-09-15 Published:2014-09-01

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摘要： 目的 探讨淋球菌rpsE基因突变与淋球菌大观霉素耐药的相关性。 方法 对临床分离的4株大观霉素耐药的淋球菌株（MIC128 μg/ml、256 μg/ml）的rpsE基因进行PCR扩增测序分析，寻找可能的突变位点，通过DNA转化技术将含有突变基因的细菌基因组DNA转化入敏感的淋球菌株，检测转化成功的淋球菌的MIC并进行PCR扩增测序，分析发现的突变位点与淋球菌大观霉素耐药的相关性。 结果 4株大观霉素耐药的菌株均发现rpsE基因A70C（Thr24Pro）突变，而16S rRNA大观霉素耐药决定区（SRDR）未发现任何突变；大观霉素敏感菌株的16S rRNA和rpsE基因均未发现突变。转化后获得的大观霉素耐药淋球菌中亦发现了同样的rpsE基因突变。 结论 rpsE基因单点突变与淋球菌对大观霉素耐药相关。

关键词: 淋病奈瑟球菌, 基因，rpsE, 点突变, 大观霉素, 抗药性

Abstract: Zhu Wen*, Jiang Faxing, Su Xiaohong, Le Wenjing, Wang Na. *Department of Dermatology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, China Corresponding authors: Jiang Faxing, Email: jiangfxing@126.com; Su Xiaohong, Email: suxhong@yahoo.com 【Abstract】 Objective To evaluate the relationship between spectinomycin resistance in Neisseria gonorrhoeae and mutations in the rpsE gene. Methods Genomic DNA was extracted from 4 clinical isolates of Neisseria gonorrhoeae with different levels of spectinomycin resistance. Then, PCR was performed to amplify the entire rpsE gene and the spectinomycin resistance-determining region （SRDR） in the 16S rRNA gene followed by direct sequencing. Two spectinomycin-sensitive Neisseria gonorrhoeae strains were transformed with the genomic DNA containing the mutant rpsE gene. Subsequently, the susceptibility of the transformants to spectinomycin was determined, and PCR was performed to amplify the rpsE and 16S rRNA genes in the transformants followed by sequencing. Results All the 4 spectinomycin-resistant Neisseria gonorrhoeae strains harbored an A70C transversion in the rpsE gene, but no abnormality in the SRDR of the 16S rRNA gene. No mutations were detected in the spectinomycin-sensitive Neisseria gonorrhoeae strains. The A70C transversion in the rpsE gene was also detected in the two Neisseria gonorrhoeae transformants with spectinomycin resistance. Conclusion The A70C point mutation within the rpsE gene is associated with spectinomycin resistance in Neisseria gonorrhoeae.

Key words: Neisseria gonorrhoeae, Genes, rpsE, Point mutation, Spectinomycin, Drug resistance