中华皮肤科杂志

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Kaposi肉瘤组织内人类疱疹病毒8型的K15基因型研究

张德志, 普雄明, 吴卫东, 靳颖   

  1. 新疆维吾尔自治区人民医院皮肤性病, 乌鲁木齐830001
  • 收稿日期:2006-12-08 出版日期:2007-11-15 发布日期:2007-11-15
  • 通讯作者: 普雄明,email:puxiongming@126.com E-mail:puxiongming@126.com

Genotyping of K15 gene of human herpesvirus 8 in Kaposi's sarcoma

ZHANG De-zhi, PU Xiong-ming, WU Wei-dong, JIN Ying   

  1. Department of Dermatology and Venereology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
  • Received:2006-12-08 Online:2007-11-15 Published:2007-11-15

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摘要： 目的 了解Kaposi肉瘤组织中人类疱疹病毒8型(HHV-8)K15等位基因型分布情况,并初步探讨Kaposi肉瘤不同临床分型及临床表现与不同HHV-8 K15等位基因型的相关性.方法 采用酚-氯仿-异戊醇法对收集的27例Kaposi肉瘤石蜡包埋组织标本进行病毒DNA抽提,并使用巢式PCR扩增K15基因片段,然后测序并确定其等位基因型.结果 27例Kaposi肉瘤中有22例HHV-8感染为阳性,阳性率为81.48%,其中4例艾滋病-Kaposi肉瘤患者HHV-8感染均为阳性,感染率100%;在分析的22例HHV-8病毒株中,20例为P型,2例为M型;4例艾滋病-Kaposi肉瘤患者感染的均为P型HHV-8,2例M型感染者均为经典型Kaposi肉瘤患者.结论 Kaposi肉瘤组织内HHV-8的K15等位基因型主要是P型,也存在部分M型HHV-8感染者.4例艾滋病-Kaposi肉瘤患者感染的HHV-8均为P型.

关键词: 肉瘤, 卡波西, 疱疹病毒8型, 人, 基因, K15, 等位基因

Abstract: Objective To study the distribution of K15 alleles of human herpesvirus 8 (HHV-8) in Kaposi's sarcoma (KS) in Xinjiang,and to investigate the relationship between clinical profiles of KS and alleles of HHV-8 K15.Methods HHV-8 DNA was extracted with phenol-chloroform-isoamyl alcohol from 27 formalin-fixed,paraffin-embedded tissue specimens of KS.The HHV-8 K15 gene was amplified by nestedPCR,and sequenced for the identification of K15 allele.Results HHV-8 DNA could be detected in 22 (81.48%) out of 27 KS patients in Xinjiang.HHV-8 DNA was detected in all 4 patients with AIDS-related KS.Twenty viral strains were identified as P type,including all 4 from the AIDS-related KS patients;two strains were identified as M type,which were all from the classical KS patients.Conclusions In KS,most of HHV-8 K15 alleles are P type,and some are M type.The 4 patients with AIDS-related KS all carried P type of K15 allele.

Key words: Sarcoma,Kaposi, Herpesvirus 8,human, Gene,K15, Alleles