中华皮肤科杂志 ›› 2007, Vol. 40 ›› Issue (3): 136-138.

• 论著 • 上一篇    下一篇

镍接触性皮炎皮损趋化因子及其受体的研究

窦侠, 刘玲玲, 闻卫兢, 朱学骏   

  1. 北京大学第一医院皮肤科, 100034
  • 收稿日期:2006-04-18 出版日期:2007-03-15 发布日期:2007-03-15
  • 作者简介:窦侠,email:douxia@medmail.com.cn

Expression of chemokines and their receptors in lesions of nickel elicited contact dermatitis

DOU Xia, LIU Ling-ling, WEN Wei-jing, ZHU Xue-jun   

  1. Department of Dennatology, First Hospital of Peking University, Beijing 100034, China
  • Received:2006-04-18 Online:2007-03-15 Published:2007-03-15

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摘要： 目的 探讨趋化因子及其受体在镍接触性皮炎发病中的作用.方法 留取镍接触性皮炎患者直接接触部位皮损和部分患者非直接接触部位皮损进行趋化因子受体CXCR3和CCR4免疫组化染色,同时采用RT-PCR分析HaCaT细胞经硫酸镍刺激后γ干扰素诱导蛋白-10(IP-10)、干扰素诱导T细胞趋化因子α(I-TAC)、胸腺活化调节的趋化因子(TARC)和巨噬细胞来源趋化因子(MDC)在mRNA水平的表达情况.结果 镍接触性皮炎患者皮损中浸润细胞以CD45Ro+CD4+T细胞为主,CD8+细胞比例小于25%;直接接触与非直接接触部位皮损浸润细胞中50%以上CXCR3染色阳性,CCR4阳性细胞少于20%.HaCaT细胞经硫酸镍刺激后主要表达趋化因子IP-10和I-TAC,48～72 h最明显.结论 镍所致系统性接触性皮炎患者非直接与直接接触部位皮损在T细胞免疫反应的效应阶段炎症反应类型基本相同.

关键词: 皮炎,接触性, 镍, 炎症趋化因子类, 受体,趋化因子

Abstract: Objective To study the role of chemokines and their receptors in the pathogenesis of nickel elicited contact dermatitis.Methods The expressions of chemokine receptors, including CXCR3 and CCR4, were detected by immunohistochemistry in primary and secondary lesions of nickel elicited contact dermatitis.RT-PCR was used to measure the mRNA expression of different types of chemokines, including interferon-y-induced protein 10(IP-10),interferon-inducible T cell α-chemoattractant(I-TAC),thymus and activation regulated chemokine(TARC)and macrophage derived chemokine(MDC),in cultured human keratinocyte cell line HaCaT after treated with nickel sulfate.Results In the lesions of nickel dermantis, CD45RO+CD4+ T cells were the predominant infiltrating cells, whereas CD8+ cells were less than 25%, and more than 50% infiltrating cells were positive for CXCR3, while less than 20% for CCR4.RT-PCR results showed that afrer being treated with nickel sulfate HaCaT cells mainly expressed IP-10 and I-TAC, which are Thl associated chemokines, and the peak expression was observed at 48 h for IP-10 and 72 h for I-TAC.Conclusion The inflammation type at the effector phase of T cell immune response in primary lesions may be identical to that in secondary lesions of nickel elicited contact dermatitis.

Key words: Dermatitis, contact, Nickel, Chemokines, Receptors, chemokine