中华皮肤科杂志 ›› 2007, Vol. 40 ›› Issue (3): 133-135.

• 论著 •    下一篇

南京地区1999-2004年四环素高度耐药淋球菌tetM基因分型及流行研究

蒋法兴, 其木格, 戴秀芹, 孙厚华, 叶顺章, 苏晓红   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所, 南京, 210042
  • 收稿日期:2006-03-09 出版日期:2007-03-15 发布日期:2007-03-15
  • 通讯作者: 苏晓红,email:suxhong@yahoo.com E-mail:suxhong@yahoo.com

Molecular epidemiology of tetM gene in high-level tetracycline-resistant Neisseria gonorrhoeae isolates in Nanjing, 1999-2004

JIANG Fa-xing, Qi Mu-ge, DAI Xiu-qin, SUN Hou-hua, YE Shun-zhang, SU Xiao-hong   

  1. Institute of Dernaatology, Chinese Academy of Medical Sciences & Peking Medical College, Nanjing 210042, China
  • Received:2006-03-09 Online:2007-03-15 Published:2007-03-15

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摘要： 目的 了解南京地区四环素高度耐药淋球菌(TRNG)tetM基因的分子流行病学情况.方法 采用琼脂稀释法检测菌株是否为TRNG,采用纸片酸度定量法测定菌株是否产β-内酰胺酶.采用单管PCR方法对TRNG阳性菌株作tetM基因分型.结果 1999-2004年间南京地区804株淋球菌中共检出136株(16.92%)TRNG,β-内酰胺酶阳性的淋球菌(PPNG)为290株,所有TRNG中69.85%(95/136)为PPNG/TRNG,TRNG的阳性率逐年增高.tetM基因分型结果显示,荷兰变型为135株,美国变型仅1株.结论 南京地区流行的TRNG以荷兰型tetM基因为主(99.26%),美国型仅为偶发.

关键词: 中毒性表皮松解症, stevens-Johnson综合症, 皮质类固醇激素, 静脉注射丙种球蛋白, 联合治疗, SCORTEN, 奈瑟球菌,淋病, 四环素抗药性, tetM基因

Abstract: Objective To investigate the molecular epidemiology of tetM gene in Neisseria gonorrhoeae strains with high level resistance to tetracycline(TRNG)in Nanjing.Methods TRNG were detected by an agar dilution method.β-lactamase production was examined by paper acidometric testing.A single tube PCR was used to subtype tetM gene in TRNG.Results The isolation rate of TRNG increased year by year from 1999 to 2004, and a total of 136(16.92%)TRNG strains were isolated out of 804 Neisseria gohorrhoeae strains in Nanjing during this period.Of these TRNG, 69.85%(95/136)produced β-lactamase (PPNG/TR'VG).Subtyping of tetM gene showed that 135 TRNG were Dutch variant, while only one was American variant.Conclusion In Nanjing, Dutch variant is the predominant tetM determinant in TRNG, whereas American variant is the sporadic determinant.

Key words: Neisseria gonorrhoeae, Tetracycline resistance, tetM gene