中华皮肤科杂志

• 论著 • 上一篇    下一篇

小檗碱衍生物HB-13体外抗单纯疱疹病毒的研究

吴建兵, 李新宇, 林麟, 崔盘根, 郑家润   

  1. 中国医学科学院皮肤病研究所, 南京210042
  • 收稿日期:2006-01-05 出版日期:2007-11-15 发布日期:2007-11-15
  • 通讯作者: 郑家润,email:jrzheng@jsmail.com.cn E-mail:jrzheng@jsmail.com.cn

In vitro Antiviral activity of a berberine derivant HB-13 against herpes simplex virus

WU Jian-bing, LI Xin-yu, LIN Lin, CUI Pan-gen, ZHENG Jia-run   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Nanjing 210042, China
  • Received:2006-01-05 Online:2007-11-15 Published:2007-11-15

PDF (PC)

92

赞

摘要： 目的 探讨小檗碱衍生物HB-13及其前体小檗碱体外抗HSV-1和HSV-2的活性.方法 利用Vero细胞体外培养模型,以阿昔洛韦(ACV)为对照药,通过观察细胞病变效应,检测HB-13和小檗碱的细胞毒性、HSV-1和HSV-2液的病毒滴度及药物抑制病毒致细胞病变效应.结果 HB-13、小檗碱和阿昔洛韦的半数中毒质量浓度(TC50)分别为31.99,380和>800 μg/mL;对HSV-1的半数抑制质量浓度(IC50)分别为1.328,>100和0.443 μg/mL,治疗指数分别为24.09,<3.80和>1805.87;对HSV-2的IC50分别为1.344,>100和0.679μg/mL;治疗指数分别为23.80,<3.80和>1178.20.结论 HB-13具有明显的抗HSV作用,而小檗碱无实用抗HSV活性.

关键词: 单纯疱疹病毒属, 体外实验, 小檗碱, HB-13

Abstract: Objective To evaluate the in vitro antiviral activity of HB-13,a compound derivant from berberine and its prodrug berberine,against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). Methods Vero cells were cultured in vitro and infected with HSV.Then,various concentrations of HB-13, berberine,and aciclovir were used to treat these infected cells.The cytopathic effect was observed to determine the antiviral effects and cytotoxicity of HB-13 and berberine.Results For HB-13,berberine and acyclovir,the half toxicity concentration (TC50) to Vero cells was 31.99,380 and more than 800μg/mL, respectively;the average half inhibitory concentration (IC50) against HSV-1 was 1.328,more than 100,and 0.443μg/mL,respectively,the treatment index (TI) against HSV-1 was 24.09,less than 3.80,and more than 1805.87,respectively;the IC50 against HSV-2 was 1.344,more than 100,and 0.679μg/mL,respectively,the TI against HSV-2 was 23.80,less than 3.80 and more than 1178.20,respectively.Conclusion HB-13 possesses marked antiviral activity against HSV-1 and HSV-2 in vitro,while berberine does not.

Key words: Simplexvirus, In vitro, Berberine, HB-13