中华皮肤科杂志 ›› 1993, Vol. 26 ›› Issue (1): 26-28.

• 论著 • 上一篇    下一篇

间接酶标葡萄球菌A蛋白染色法测定人表皮细胞ABH血型抗原

赵阳兵, 赵雄飞, 路淑珍, 王旭   

  1. 第三军医大学烧伤研究所, 重庆 630038
  • 收稿日期:1991-11-01 修回日期:1992-04-06 出版日期:1993-02-15 发布日期:1993-02-15

Expression of ABH Blood Croup Antigens by Human Shin and Cultured Epidermis weth Indirect Enzyme Conjugated-SPA Method

ZHAO Yang-Bing, ZHA0 Xiong-Fei   

  1. Burn Reszarch Institute, South- Westen: Hospital, Chongqing 660038
  • Received:1991-11-01 Revised:1992-04-06 Online:1993-02-15 Published:1993-02-15

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摘要： 用间接酶标葡萄球菌A蛋白(SPA)染色法对人正常皮肤及培养表皮细胞的ABH血型抗原的表达进行观察.在正常皮肤,该类抗原主要分布在表皮颗粒层及棘层和真皮毛细血管内皮.培养的新生表皮均可查到与供皮者相一致的血型抗原,但其表达明显弱于正常皮肤表皮,提示表皮细胞在体外培养增殖过程中,其ABH血型抗原的表达减弱.

关键词: 上皮细胞, ABH血型坑原, 酶标SPA

Abstract: An indirect enzyme conjugated-SPA method with anti-blood group ABH McAb was utilized to detect ABH blood group antigen expression in lmman shin and cultured epidermis in this study.

Key words: Epidermal cell, ABH blood group antigen, Enzyme conjugated-SPA