中华皮肤科杂志 ›› 1992, Vol. 25 ›› Issue (6): 369-371.

• 论著 • 上一篇    下一篇

细胞培养法检测泌尿生殖系统沙眼衣原体感染

江玎妍1, 蒋文玲1, 李质怀1, 陈泽波1, 张木友2, 曾允康3, 杨永珍3, 陈秀廉4   

  1. 1. 广东省老年医学研究所病毒室, 广州 510802;
    2. 广东省皮肤病研究所性病防治中心;
    3. 广州市东山区人民医院:
    4. 广东省佛山市中医院妇产科
  • 收稿日期:1991-08-10 修回日期:1991-10-28 出版日期:1992-12-15 发布日期:1992-12-15

Detection of Urogenital Chiamydia Travchomatis Infectin with Cell Culture

WANG Ding-Yan1, JIANG Wen-Ling1   

  1. Virology Lab. Guangdong Provincial Insitute of Geratric Medicine, Guangzhou 510080
  • Received:1991-08-10 Revised:1991-10-28 Online:1992-12-15 Published:1992-12-15

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摘要： 1990年6月至1991年6月取得泌尿生殖道标本836份.用放线菌酮处理的McCoy细胞进行分离培养沙眼衣原体单克隆抗体鉴定,女性标本阳性率为8.4%(53/631),男性标本阳性率14.6%(30/205).本实验用24孔培养板代替了放有盖玻片的玻璃瓶,从而节省材料提高效率,是值得推广的筛选技术.用细胞培养免疫荧光法检测的阳性率是细胞培养碘染法的2-3倍,其差异有非常显著意义(P<0.005).

关键词: 沙眼衣原体, 衣原休感染

Abstract: Between June 1990 and June 1991,we examined 836 specimens from patients attending gynaecological out-patient clinic and sexually transmissible disease clinic of some hospitals in Guangdong.The specimens were inoculated in eycloheximide-treated MeGay cells at 37℃,5% CO2 for 48 hours and then the chlamydia trachomatis antigen ivcre detected by fluorescence labeled monoclonal antibodies(Micro Trak Syva).The positive rate far chlamydia trachomatis from female specimens(endocervical swabs) was 8.4%(53/631),the positive rate for male specimens(uragenital swabs) was 14.6%(30/205).We used a 24-well tissue culture plate for thr isolation of chlamydia trachomatis instead of flat-hottom vials containing a cover slip.The positive rate of immunafiuorescence staining is 2-3 times higher than that of iodine staining,the difference is very significant(P<0.005).

Key words: Chlamydia, trachomatis, Chlamydial infections