中华皮肤科杂志 ›› 1992, Vol. 25 ›› Issue (6): 363-365.

• 论著 •    下一篇

白念珠菌种特异性DNA探针的制备与鉴定

梁龙1, 叶庆佾1, 谭骏2   

  1. 1. 第三军医大学第一附属医院皮肤科;
    2. 第三军医大学遗传学教研室
  • 收稿日期:1991-08-29 修回日期:1992-03-12 出版日期:1992-12-15 发布日期:1992-12-15

Preparation and Ideatification of Candida albicans Species-specific DNA Probe

LIANG Long1, YE Qing-Yi1   

  1. The First Hospital, Third Military Medical Colege, Chongqing 630038
  • Received:1991-08-29 Revised:1992-03-12 Online:1992-12-15 Published:1992-12-15

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摘要： 应用DNA克隆技术成功地克隆到经EcaRI消化的自念珠菌(白念)2.6kb片段并标记成探针,与数种念珠菌及人白细胞IAN人在NC膜上作斑点杂交发现:此探针只能与白念珠菌及类星型念珠菌杂交呈强阳性,与其它念珠菌及人白细胞DNA杂交均阴性,提示可用于白念珠菌病原体的检溯及菌种的鉴定,并为系统性念珠菌病基因诊断的研究打下基础.

关键词: 自念珠菌, DNA探针, 分子克隆, 地高辛-dUTP, DNA标记

Abstract: An attempt is made to produce DNA probe that only hybridizes with the DNA from Candida albicans.Total DNr1 was iaolated from the cells of Candida albicans and digested with EcoRI restriction enzyme.A fragmont(2.6 Kb) was cloned into the EcoRI site of the plasmid vector pBR322 and replicated in E.coli K802.The candidal fragment was labelled with digoxijenin-dUTP(DigdUTP) by random primed DNA labelling method and used to probe dot blots of DNA from several Candida spacious and human leukocytes.Only the DNA from Candida albicans and a strain of Candida steliatoidea was found to contain sedtrences of significant homology for hybridization.From tlue above results,it is concluded that this cloned fragment may passibly be used as a DNA probe for detecting and identifying the presence of Candida albicans.

Key words: Candida albicans, DNA probes, Molecular coning DigoxigenindUTP, DAN labeling