中华皮肤科杂志 ›› 1990, Vol. 23 ›› Issue (1): 19-21.

• 论著 • 上一篇    下一篇

体外培养人胚雪旺氏细胞及其对麻风菌吞噬的观察

丘钜世1, 刘子君1, 吉重敏1, 张萌1, 丘红梅1, 方郭锡2   

  1. 1. 中山医科大学病理教研室;
    2. 广州市皮肤病防治所
  • 收稿日期:1988-10-13 修回日期:1989-03-02 出版日期:1990-02-15 发布日期:1990-02-15
  • 基金资助:
    自然科学基金资助课题之一

OBSERVATION ON PHAGOCYTOSIS AND REACTIVITY TO M.LEPRAE BY CULTURED HUMAN SCHWANN CELLS

QIU Ju-Shi1, LIU Zi-Jun1   

  1. Department of Pathology, Zhongshan Medical University, Guangzhou
  • Received:1988-10-13 Revised:1989-03-02 Online:1990-02-15 Published:1990-02-15

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摘要： 体外培养了6例人胚外周神经的雪旺氏细胞,均经传代繁殖,1例传至第11代,存活期达110天.雪旺氏细胞与麻风杆菌混合培养,定期取出生长于盖玻片上的细胞进行抗酸染色观察,感染10小时后,可见少量(15%左右)细胞吞噬了抗酸杆茵,菌多位于细胞浆的外周部;以后吞菌细胞数逐渐增多且向细胞浆的中央部分推移,72小时后吞苗细胞数达到高峰(95%以上),在细胞内可形成菌团或菌球.电镜下雪旺氏细胞表面的微绒毛间及胞浆内见有多量麻风菌.96小时后,雪旺氏细胞出现明显变性及坏死,但细胞内的麻风杆菌却无明显改变.

Abstract: The Schwann cells taken from the peripheral nerves of 6 human fetuses were cultured and subcultured.Among which the Schwann cells of 1 fetus could 6e subcuitured up to 11th generation covering more than 110 days.The Schwann cells and M.Leprae were co-cultuned by coverslip method,and the coverslips were stained (acid-fast stain) anal the phagocytosis of M.Leprae by Schwann cells was observed under light microscopy in regular intervals.About 15% of Schwann cells phagocytosed M.Leprae 10 ours after infection.Later on the number of the cells phagocytosed M.Leprae steadily increased and reached to the peak (95%) of Phagocytic index 72 hours after co-culature.M.Leprae giobi could aslo be found in Schwann cells.By electron microscopy many M.Leprae could be observed among the microvilli as well as in the cytoplasm of the infected Schwann cells.96 hours after infection the Schwainn cells which phagocytased many M.Leprae underwent degeneration and necrosis,but the M.Leprae in the cells still existed with their morphology unchanged.