中华皮肤科杂志 ›› 1989, Vol. 22 ›› Issue (2): 92-94.

• 论著 • 上一篇    下一篇

恶性黑素瘤铁蛋白、α1-抗胰糜蛋白酶和S100蛋白的免疫组化研究

丁华野, 李青, 刘彦仿   

  1. 第四军医大学病理学教研室
  • 收稿日期:1987-08-25 修回日期:1988-01-14 出版日期:1989-04-15 发布日期:1989-04-15

IMMUNOHISTOCHEMICAL STUDY OF FE, S100 PROTEIN AND A1Achy IN MALIGNANT MELANOMA

DING Hua-Ye, LI Qing   

  1. The Fourth Military Medical College, Xi-an
  • Received:1987-08-25 Revised:1988-01-14 Online:1989-04-15 Published:1989-04-15

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摘要： 作者采用双PAP法,对22例福尔马林固定,石蜡包埋的恶性黑素瘤(MM)组织进行了铁蛋白(FE)、S100蛋白和α1一抗胰糜蛋白酶(A1Achy)的免疫组织化学定位研究,同时观察了MM的甲醛诱发荧光(FIF),其阳性率分别为77.3%(FE)、95.5%(S100蛋白)、63.6%(A1Achy)和100%(FIF).结果提示1,MM患者血清FE升高可能由于瘤细胞合成增加、间质反应和单核巨噬细胞系统的非特异性反应所致.2.S100蛋白及FIF有助于低分化MM(特别是无黑素性MM)和皮肤Paget病及某些组织起源不明的肿瘤鉴别诊断.

Abstract: Twenty-two case of malignant melanoma (MM) were studied immunohistochemically for the presence of ferritin (FE),S100 protein and alpha-1-antichymotrypsin (AlAchy) by the double PAP method on routine formalin fixed and paraffin-embedded tissues.The formaldehyde-induced fluorescence (FIF) in MM was also observed.It was found that the positive rates of FE,S100 protein,A1Achy and FIF in these cases were 77.3%,95.5%,63.3% and 100% respectively.The findings suggest that:1.raised serum FE concentration in MM patients may be attri buted to the increase of FE synthesis in tumor cells,stromal reaction and non-specific response of mononu-clear phagocytic system;2.detection of S100 protein and FIF may help to distinguish poorly differentiated MM,especially the amelanotic type,from Pnget's disease of the skin and some tumors of obscure histogenetic origin.