中华皮肤科杂志

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奥曲肽对系统性红斑狼疮患者体外培养单一核细胞增殖及分泌细胞因子的影响

黄雯, 黄岚, 李峰, 冯树芳, 徐金华   

  1. 复旦大学附属华山医院皮肤科, 上海200040
  • 收稿日期:2005-11-28 出版日期:2006-04-15 发布日期:2006-04-15
  • 通讯作者: 徐金华,email:xjhhs@medmail.com.cn E-mail:xjhhs@medmail.com.cn
  • 基金资助:
    国家自然科学基金(39970688)

Effect of octreotide on the secretion of cytokines by and proliferation of cultured PBMCs from patients with systemic lupus erythematosus

HUANG Wen, HUANG Lan, LI Feng, FENG Shu-fang, XU Jin-hua   

  1. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2005-11-28 Online:2006-04-15 Published:2006-04-15

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摘要： 目的 探讨奥曲肽对SLE患者体外培养单一核细胞增殖及其分泌细胞因子的影响。方法 对SLE患者和正常人对照的单一核细胞进行体外培养,用MTT法测定单一核细胞加入不同浓度奥曲肽后的细胞增殖率,计算不同浓度奥曲肽对单一核细胞的抑制率,同时以生长激素加奥曲肽进行干预,并测定细胞因子的分泌水平。结果 0.1 ng/mL奥曲肽对单一核细胞增殖无抑制作用,1 ng/mL奥曲肽开始有微弱的抑制作用,10 ng/mL奥曲肽有明显的抑制作用,且呈剂量依赖性。SLE患者外周血单一核细胞在体外经奥曲肽干预后,其IL-6、IL-10和IFN-γ分泌量较未处理组显著下降,生长激素加奥曲肽共同干预后的分泌量较生长激素干预的分泌量显著下降。结论 奥曲肽体外能抑制单一核细胞增殖,且能抑制生长激素诱导的细胞因子的分泌。

关键词: 红斑狼疮, 系统性, 奥曲肽, 生长激素, 白细胞介素6, 白细胞介素10, 干扰素Ⅱ型

Abstract: Objective To investigate the effect of octreotide (OCT) on the proliferation capacity of and secretion of cytokines by cultured PBMCs from patients with systemic lupus erythematosus (SLE). Methods PBMCs from patients with SLE and healthy controls were cultured in vitro with various concentrations of OCT,pretreated with or without growth hormone (GH).The proliferation capacity of PBMCs was detected by MTT method,and the secretion of cytokines by PBMCs was measured with ELISA.Results OCT could inhibit the proliferation capacity of PBMCs in a dose dependent manner:0.1 ng/mL of OCT had no effect on the proliferation,1 ng/mL OCT had a weak inhibitory effect,and 10 ng/mL OCT had a marked inhibitory effect.The secretion of IL-6,IL-10 and IFN-γby PBMCs was significantly decreased with the treatment of OCT,and was also significantly decreased with the co-treatment of GH and OCT compared to that with the treatment of GH only.Conclusion OCT can inhibit the proliferation capacity of PBMCs and secreation of IL-6,IL-10 and IFN-γby PBMCs induced by GH.

Key words: Lupus erythematosus, systemic, Octreotide, Growth hormone, Interleukin-6, Interleukin-10, Interferon typeⅡ