中华皮肤科杂志

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IFI 16及其信号转导途径在系统性红斑狼疮发病中的作用

徐祖森, 周映   

  1. 华中科技大学同济医学院附属同济医院皮肤科, 武汉430030
  • 收稿日期:2005-12-12 出版日期:2006-04-15 发布日期:2006-04-15

The role of interferon inducible protein IFI 16 and JAK-STAT pathway in the pathogenesis of systemic lupus erythematosus

XU Zu-sen, ZHOU Ying   

  1. Department of Dermatology, TongJi Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
  • Received:2005-12-12 Online:2006-04-15 Published:2006-04-15

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摘要： 目的 探讨干扰素诱导蛋白IFI 16及其Janus激酶/信号转导和转录激活因子(JAK-STAT)信号转导途径在系统性红斑狼疮(SLE)发病中的作用。方法 逆转录-聚合酶链反应(RT-PCR)检测35例SLE患者和16例正常人外周血单一核细胞(PBMC)中IFI 16的表达水平。体外培养PBMC,用JAK2抑制剂AG490抑制JAK-STAT信号转导途径,再用RT-PCR检测PBMC中IFI 16的表达水平。结果 与对照组比较,活动期、非活动期组IFI 16 mRNA的表达水平明显增高,差异均有统计学意义(P<0.001)。而活动期与非活动期组比较,差异无统计学意义(P>0.05)。在用AG490抑制JAK-STAT信号转导途径后,活动期与非活动期组PBMC中IFI 16 mRNA的表达水平均明显下降,差异均有统计学意义(P<0.001),而正常组用药前后比较差异无统计学意义(P>0.05)。结论 IFI 16在SLE患者的异常高表达提示IFI 16基因可能为SLE的致病基因之一。

关键词: 红斑狼疮, 系统性, 干扰素诱导核蛋白16, JAK-STAT信号转导途径

Abstract: Objective To investigate the role of interferon inducible protein IFI 16 and JAK-STAT pathway in the pathogenesis of systemic lupus erythematosus(SLE).Methods The peripheral blood mononuclear cells (PBMCs) isolated from 35 SLE patients and 16 normal controls were subjected either to detection of the mRNA expression of IFI16,or to culture in vitro,pretreated with AG490 to block the JAK-STAT pathway,and then deteced by RT-PCR for the expression of IFI 16 mRNA.Results The mean expression level of IFI 16 mRNA was significantly higher in both active SLE group and inactive SLE group than that in normal controls (P<0.001).No statistical difference was found in IFI 16 mRNA expression between the active and inactive SLE group (P>0.05).After the JAK-STAT pathway was blocked by AG490,the expression of IFI 16 mRNA were significantly decreased in active and inactive SLE group (P<0.001),but remained unchanged in normal controls (P>0.05).Conclusion The overexpression of IFI 16 gene in patients with SLE indicates that it might be a causative gene of SLE.

Key words: Lupus erythematosus, systemic, IFI 16, JAK-STAT pathway