中华皮肤科杂志

• 论著 • 上一篇    下一篇

Stat1和含SH2区域的SHP-1在尖锐湿疣中的表达

陶小华1, 董玉娥1, 孟群2, 潘卫利1, 程浩3   

  1. 1. 浙江省人民医院皮肤科, 杭州310014;
    2. 浙江省人民医院病理科, 杭州310014;
    3. 浙江大学附属邵逸夫医院皮肤科
  • 收稿日期:2005-12-06 出版日期:2006-04-15 发布日期:2006-04-15
  • 基金资助:
    浙江省卫生厅资助课题(20005A06)

Expression of signal transducers and activators of transcription 1 and Src-homology 2 domain phosphatase1 in condyloma acuminatum

TAO Xiao-hua1, DONG Yu-e1, MENG Qun2, PAN Wei-li1, CHENG Hao3   

  1. Department of Dermatology, Zhejiang Provincial People's Hospital, Hangzhou 310014, China
  • Received:2005-12-06 Online:2006-04-15 Published:2006-04-15

PDF (PC)

89

赞

摘要： 目的 探讨JAK/STAT通路中信号转导和转录激活因子Stat 1和含SH2区域的酪氨酸磷酸酶SHP-1在尖锐湿疣中的异常表达。方法 原位杂交技术诊断HPV6/11相关尖锐湿疣,SP免疫组化染色技术检测40例HPV6/11阳性尖锐湿疣患者皮损、20例正常人皮肤(包皮)中Stat 1和SHP-1的表达及分布。结果 尖锐湿疣皮损中Stat 1和SHP-1的表达均强于正常人上皮中的表达,两者比较差异均有统计学意义(前者W=382.5,P<0.01;后者W=369,P<0.01)。尖锐湿疣患者皮损中Stat 1和SHP-1阳性细胞主要分布于棘细胞层,Stat 1阳性反应定位于胞浆或胞核,SHP-1阳性反应主要定位于胞浆。尖锐湿疣患者中Stat 1和SHP-1的表达呈显著相关性(tau-b=1.0,P<0.01)。结论 HPV6/11阳性尖锐湿疣中Stat 1和SHP-1表达增强,提示HPV6/11感染时可能通过JAK/STAT通路,在角质形成细胞过度增殖中起作用。

关键词: 尖锐湿疣, 乳头状瘤病毒, 人, 蛋白质酪氨酸磷酸酶, 信号转导和转录激活因子

Abstract: Objective To investigate the abnormal expression of signal transducers and activators of transcription 1(Stat 1)and Src-homology 2 domain phosphatasel(SHP-1)in JAK/STAT route of condyloma acuminatum(CA)and its significance.Methods HPV6/11 related CA was diagnosed by in situ hybridization;the expression and distribution of Stat 1 and SHP-lwere examined by SP immunohistochemistry in 40 specimens from CA patients positive for HPV 6/11 and 20 normal skin specimens (foreskins).Results The expression of both Stat 1 and SHP-1 was significantly higher(both P<0.01) in CA lesions than that in normal epithelium.The Stat 1-and SHP-1-positive cells were mainly distributed in prickle layer of HPV 6/11 related CA lesions.The positive signal of Stat 1 was located in the cytoplasm or nuclei,and that of SHP-1 was mainly distributed in the cytoplasm.There was a significant correlation(P<0.01)between the expression of Star 1 and that of SHP-1 in HPV 6/11-related CA lesions.Conclusion The expression of Stat 1 and SHP-1 is increased in HPV6/11 positive CA lesions,which suggests that HPV6/11 may play a role in the over-proliferation of keratinocytes through JAK/STAT route.

Key words: Condylomata acuminata, Papillomavirus, human, Protein-tyrosine-phosphatase, Signal transducers and activators of transcriptionl