应用单克隆抗体研究寻常型银屑病患者外周血中的T细胞及其亚群

中华皮肤科杂志 ›› 1986, Vol. 19 ›› Issue (2): 70-72.

应用单克隆抗体研究寻常型银屑病患者外周血中的T细胞及其亚群

强能贤, 钟铭英, 刘训荃, 徐清美, 金婉清, 李涛

中国医学科学院皮肤病研究所

收稿日期:1985-06-24 修回日期:1985-09-14 出版日期:1986-04-15 发布日期:1986-04-15

Study of T cells and their subpopulations in peripheral blood of patients with psoriasis wlgaris using monoclonal antibodies

Qiang Nengxian

Received:1985-06-24 Revised:1985-09-14 Online:1986-04-15 Published:1986-04-15

摘要/Abstract

摘要： 以抗人T细胞表面坑原的单克隆抗体OKT3、OKT4和OKT8用直接免疫荧光法分析了进行期、静止期和退行期寻常型银屑病(各10例)患者外周血中的总T细胞、协助/诱导性T细胞和抑制/细胞毒性T细胞的百分数、以及OKT4⁺/OKT8⁺细胞的比率.10名正常健康人为对照组.结果发现,与健康人对照组比较,各期寻常型银屑病患者的OKT3⁺,OKT4⁺,OKT8⁺细胞数和OKT4⁺/OKT8⁺细胞的比率无显著差异(P>0.05),各期寻常型银屑病患者间亦无显著性差异(P>0.05).最后,对本研究结果进行了讨论,认为银屑病中所见的T细胞、抑制性和协助性T细胞亚群数的异常可能是一种继发现象.

Abstract: Monoclonal antibodies of anti-human T cell surface antigens, OKT3, OKT4 and OKT8 were used to determine the percentages of total T, T helper/inducer and T suppressor/cytoto-xic cells, and the ratios of OKT4⁺/OKT8⁺ cells in the peripheral blood of the patients with psoriasis vulugaris by direct immunofluorescent method. The patients were divided into 3 groups, active, resting and regressive stages. Each group consisted of 10 cases. Controls consisted of 10 healthy adults. Compared with the control group, no significant differences were found,in the percentages of OKT3⁺, OKT4⁺ and OKT8⁺ cells and the ratios of OKT4⁺/OKT8⁺ cells of the various stage groups (P > 0.05). It also showed no significant differences between the various stage groups (P > 0.05). Finally, the authors suggest that the quantitative abnormalities of T cells and their subpopulations, T suppressor and T helper cells may be secondary in the patients with psoriasis.

强能贤, 钟铭英, 刘训荃, 徐清美, 金婉清, 李涛. 应用单克隆抗体研究寻常型银屑病患者外周血中的T细胞及其亚群[J]. 中华皮肤科杂志, 1986, 19(2): 70-72.

Qiang Nengxian. Study of T cells and their subpopulations in peripheral blood of patients with psoriasis wlgaris using monoclonal antibodies[J]. Chinese Journal of Dermatology, 1986, 19(2): 70-72.