中华皮肤科杂志 ›› 1986, Vol. 19 ›› Issue (2): 63-66.

• 论著 •    下一篇

鼠麻风分枝菌于鼠腹腔巨噬细胞内的体外培养

李伏田1, 柯国芳1, 刘明2   

  1. 1. 上海市遵义医院;
    2. 卫生部上海生物制品研究所
  • 收稿日期:1985-05-30 修回日期:1985-09-02 出版日期:1986-04-15 发布日期:1986-04-15

Multiplication of mycobacterium lepraemurium in mouse peritoneal macrophages in vitro

Li Futian1   

  • Received:1985-05-30 Revised:1985-09-02 Online:1986-04-15 Published:1986-04-15

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摘要： 应用含40%猪血清的199液能支持鼠腹腔巨噬细胞的长期体外培养.接种鼠麻风菌后的2~4周内,细菌增长迟缓,以后进入对数期,对数期细菌倍增时间的95%可信限为4.9~5.5天.迟缓期的出现与细胞内触性磷酸酶活性和细胞培养期龄无关.传代培养无迟缓期.利福平和链霉素能抑制菌在对数期的增殖.

Abstract: Mouse peritoneal macrophages survive a long period in vitro in a medium composed of TC199 supplemented with 40% pooled swine serum. A lag phase of multiplication of Mycobacterium lepraemurium in cell culture was observed during the first 2-4 weeks after inocelation, then followed with a logarithmic phase. The 95% confidence limit of doubling time of the organism in the latter phase is 4.9~5.5 days. The retardation and the rapidity of the growth of the organism during the two different phases are neither related to cellular acid phosphatase activity nor to the longevity of cells in culture. The growth of organisms is suppressed by the addition of rifampicin or streptomycin in culture medium.