PCR检测深部致病真菌感染的研究

中华皮肤科杂志

PCR检测深部致病真菌感染的研究

刘军, 沈永年, 吕桂霞, 陈伟, 刘维达

中国医学科学院、中国协和医科大学皮肤病研究所

收稿日期:2005-12-05 出版日期:2006-04-15 发布日期:2006-04-15
基金资助:
江苏省卫生厅医学科技发展基金重大课题(H200203)

Detection of deep-seated pathogenic fungi by polymerase chain reaction

LIU Jun, SHEN Yong-nian, LÜ Gui-xia, CHEN Wei, LIU Wei-da

Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China

Received:2005-12-05 Online:2006-04-15 Published:2006-04-15

摘要/Abstract

摘要： 目的探讨PCR在可疑深部真菌感染临床标本检测中的应用.方法对60份临床标本分别进行传统真菌培养以及真菌通用引物PCR和三重PCR检测.结果真菌培养阳性共19份,其中白念珠菌10份、烟曲霉2份、新生隐球菌2份、其他菌株5份(包括光滑念珠菌2份、热带念珠菌2份、克柔念珠菌1份):真菌通用PCR检测共有21份阳性,经三重PCR检测共有15份阳性,分别为白念珠菌10份、烟曲霉3份、新生隐球菌2份.PCR方法与传统培养方法相比,检出率差异无统计学意义.结论 PCR检测可疑深部真菌感染临床标本快速简便,稳定性好,敏感性和特异性均较理想,适合临床实验室常规使用.

关键词: 真菌病, 念珠菌属, 曲霉菌, 烟, 隐球菌, 新型, 聚合酶链反应

Abstract: Objective To study the clinical application of polymerase chain reaction(PCR)in the detection specimens from patients with suspected deep-seated pathogenic fungal infection.Methods Sixty clinical specimens were detected with pan-fungal PCR,triplex PCR and traditional fungal culture,respectively.Results Nineteen specimens were positive by culture,consisting of 10 strains of Candida albicans,2 strains of Aspergillus fumigatus,2 strains of Cryptococcus neoformans and 5 other strains(2 strains of Candida glabrata,2 strains of Candida tropicalis and 1 strain of Candida krusei);21 were positive by pan-fungal PCR;and 15 were positive by triplex PCR,including 10 strains of Candida albicans,3 strains of Aspergillus fumigatus and 2 strains of Cryptococcus neoformans.There was no statistical significance in the detection rate of fungi between the PCR and culture methods.Conclusion The PCR procedure is rapid,convenient,and reliable for the detection of deep-seated pathogenic fungi with higher specificity and sensitivity,and may be routinely used in clinical laboratory.

Key words: Mycoses, Candida, Aspergillus fumigatus, Cryptococcus neoformans, Polymerase Chain Reaction

刘军, 沈永年, 吕桂霞, 陈伟, 刘维达. PCR检测深部致病真菌感染的研究[J]. 中华皮肤科杂志, 2006, 39(8): 439-441.

LIU Jun, SHEN Yong-nian, LÜ Gui-xia, CHEN Wei, LIU Wei-da. Detection of deep-seated pathogenic fungi by polymerase chain reaction[J]. Chinese Journal of Dermatology, 2006, 39(8): 439-441.

参考文献

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