中华皮肤科杂志 ›› 1985, Vol. 18 ›› Issue (3): 129-130.

• 论著 •    下一篇

蜗牛酶对3种致病性真菌细胞壁作用的研究

戴文英, 孙亦新   

  1. 大连医学院附属医院皮肤科
  • 收稿日期:1984-05-11 修回日期:1984-09-04 出版日期:1985-09-15 发布日期:1985-09-15

Study on the action of snail enzyme on the cell wall of three pathogenic fungi

Dai Wenying   

  • Received:1984-05-11 Revised:1984-09-04 Online:1985-09-15 Published:1985-09-15

PDF (PC)

223

赞

摘要： 用蜗牛酶对犬小孢子菌、石膏样小孢子菌和白念珠菌进行了消化.结果50%以上菌丝细胞壁粗糙,失去折光性、变形或断裂.大分生孢子出现更多的颗粒,隔膜消失,某些细胞壁硬裂.白念珠菌仅10~%多的孢子发生改变.真菌在消化后作了4种染色.乳酸酚棉蓝比正常蓝色更深;0.5%台盼蓝液显示着色;革兰氏染色呈阴性反应;雪夫氏过碘酸糖原染色变为淡红色.

Abstract: M.caais, M. gypseum and C. albicans were digested by snail enzpme,The results showed that over 50% of the cell walls of hyphae became rough, lost refraction, deformed of broke down.In the macroconidia.the number of granules increased, the septa lost and some cell walls broke.In C.albicaas only 10-15% of spores had alteration. After digestion, they were stained with four kinds of stains: lactophenol cotton blue stained bluer than aormal, 0.5% trypan blue solution showed pigmentation. Gram's stain negative reaction and periodic acid-schiff stain reddish colour.